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| 1 | Activation of mitogen activated protein kinases via complement receptor type 2显示文摘Background Complement receptor type 2 (CR2) is the receptor for C3d and C3dg and for Epstein Barr virus The aim of our study was to explore whether CR2 can independently mediate the activation of mitogen activated protein kinases (MAPKs, including ERK, JNK, and p38MAPK), and to highlight the molecular mechanism of CD 4 + cell deletion in AIDS Methods HOS cells (HOS CR2) and HOS CD4 cells (HOS CD4CR2) stably expressing CR2 were established and then identified by FACS and Western blotting Activation and blocking tests of MAPKs were assessed by Western blot Cell proliferation was determined using Cell Titer 96 Aqueous One Solution Reagent Results FACS results showed that the positive rates of HOS CR2 and HOS CD4CR2 cells were greater than 96%, and Western blot showed that the CR2 expression levels on HOS CR2 and HOS CD4CR2 cells were high Activation and blocking tests of MAPKs (ERK, JNK, and p38MAPK) were carried out in HOS CR2, HOS CD4, and HOS CD4CR2 cells The activation of MAPKs in HOS CR2 cells stimulated with PMA (100 ng/ml) and NHS (10%) was identical The activation of MAPKs increased at 5 minutes, reached a peak at 10 minutes, and decreased to baseline within 30 minutes, all in a time dependent manner; the activation of MAPKs was blocked by anti CR2 McAb, PD98059 (inhibitor of ERK), and Wortmanin (inhibitor of PI 3K), respectively In HOS CD4 cells, MAPKs were activated by HIV gp160 In HOS CD4CR2 cells, MAPK activation was induced by HIV gp160, 10% NHS, and HIV gp160+10%NHS; phosphorylation of p38MAPK was dramatically induced by HIV gp160+NHS, and lasted for 1 hour The cell proliferation results showed that HIV gp160 inhibited the proliferation of HOS CD4 and HOS CD4CR2 cells ( P <0 01) and that NHS enhanced the effect of HIV gp160 ( P <0 01) Conclusions The activation of MAPKs is independently mediated by CR2 and that anti CR2 McAb, PD98059, and Wortmanin block the activation of MAPKs, respectively The results of the signal transduction and cell proliferation assays of HOS CD4CR2 cells show that CR2 plays a role in the pathogenesis of HIV infection, especially in the inhibition of CD 4 + cell | 罗敏华 陈明亮 Heribert Stoiber Manfred P Dierich | 2004 | Chinese Medical Journal2004，17，12： | 2 |
| 2 | Neuroinvasion by pathogens: a key role of the complement system显示文摘 | Speth C Dierich MP Gasque P | 2002 | Mol Immunol2002，38，9： | 1 |
| 3 | In vitro activity of fosfomy- cin in combination with various antistaphylococcal substances 显示文摘 | Grik K Dierich MP Pfaller K | 2001 | J Antimicrob Chemother2001，48，2： | 1 |
| 4 | Neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas显示文摘 | Gradwohl G Dierich A LeMeur M | 2000 | Proc Natl Acad Sci USA2000，97，4： | 1 |
| 5 | Cell-specificity of the chicken ovalbumin and conalbumin promoter显示文摘 | Dierich A Gaub M P Lepennec J L | 1987 | EMBO J1987，6，： | 1 |
| 6 | Strain-specific differences in the amount of Shiga toxin released from enterohemorrhagic Escherichia coli O157 following exposure to subinhibitory concentrations of antimicrobial agents显示文摘 | GRIF K DIERICH M P KARCH H | 1998 | Eur J Clin Microbiol Infect Dis1998，17，11： | 1 |
| 7 | Minimal residual disease in ovarian cancer as a target for complement-mediated mAb immunotherapy显示文摘 | BJORGE L STOIBER H DIERICH M P | 2006 | Scand J Immuno2006，63，5： | 1 |
| 8 | In vitro activity of fosfomycin in combination with various antistaphyloccal substances显示文摘 | Grif K Dierich MP Pfaller K | 2001 | J Antimicrob Chemother2001，48，2： | 1 |
| 9 | Complement in different stages of HIV infection and pathogenesis 显示文摘 | Speth C Stoiber H Dierich M P | 2003 | Int Arch Allergy Immunol2003，130，4： | 1 |
| 10 | Role of complement in the control of HIV dynamics and pathogenesis 显示文摘 | Stoiber H Speth C Dierich MP | 2003 | Vaccine2003，212，： | 1 |
| 11 | Neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas显示文摘 | Gradwohl G Dierich A LeMeur M | 2000 | Proc Natl Acad Sci USA2000，97，4： | 1 |
| 12 | Nontoxigenic sorbitol-fermenting Escherichia coli O157:NMassociated With a family Outbreak of diarrhoea显示文摘 | AllerbergefF Dierich MP Gruber MU | 2000 | Wlin Wochenschr2000，112，19： | 1 |
| 13 | Strain specific differences in the amount of Shiga toxin released from enterohemorrhagic Escherichia coli0157 following ex- posure to subinhibitory concentrations of antimicrobial agents显示文摘 | Grif K Dierich M P Karch H | 1998 | Eur J Clin Mierobioi Infect Dis1998，17，： | 1 |
| 14 | Epidemiology of infections due to multiresistantEnterobacter aerogenes in a University Hospital显示文摘 | F. Allerberger T. Koeuth C. Lass-Fl?rl M. P. Dierich C. Putensen E. Schmutzhard I. Mohsenipour H. Grundmann D. Hartung A. Bauernfeind E. Eberlein J. R. Lupski | 1996 | European Journal of Clinical Microbiology & Infectious Diseases1996，，6： | 1 |
| 15 | Incidence of fecal carriage of Listeria monocytogenes in three healthy volunteers : a one-year prospective stool survey 显示文摘 | Grif K Patscheider G Dierich M P | 2003 | European Journal of Clinical Microbiology & Infectious Diseases: Official Pubfica- tion of the European Society of Clinical Microbiology2003，22，1： | 1 |
| 16 | Oxidized LDL indaces serotonin release from blood platelets 显示文摘 | Zhao B Dierichs R Harrach-Ruprecht B | 2005 | Am J Hematol2005，48，3： | 1 |
| 17 | Intercellular adhesion molecule - 1 ( ICAM - 1 ,CD54) is associated with actin - filaments 显示文摘 | Vogetseder W Dierich MP | 1991 | Immunobiology1991，182，2： | 1 |
| 18 | Endoscopic indicators for obstructive airway complications after lung transplantation 显示文摘 | Fuehner T Dierich M Duesberg C | 2010 | Transplantation2010，90，11： | 1 |
| 19 | A mouse model for human short-stature syndromes identifies Shox2 as an up- stream regulator of Runx2 during long-bone development显示文摘 | Cobb J Dierich A H uss-Garcia Y et a l | 2006 | Proc Natl Acad Sci USA2006，103，12： | 1 |
| 20 | Variability in tellurite resistance and the ter gene cluster among Shiga toxin-producing Escherichia coli isolated from humans, animals and food 显示文摘 | ORTH D GRIF K DIERICH M P | 2007 | Res Microbiol2007，158，： | 1 |
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