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| 1 | An integrated methodology for soil moisture analysis using multispectral data in Mongolia显示文摘Soil moisture(SM)content is one of the most important environmental variables in relation to land surface climatology,hydrology,and ecology.Long-term SM data-sets on a regional scale provide reasonable information about climate change and global warming specific regions.The aim of this research work is to develop an integrated methodology for SM of kastanozems soils using multispectral satellite data.The study area is Tuv(48°40′30″N and 106°15′55″E)province in the forest steppe zones in Mongolia.In addition to this,land surface temperature(LST)and normalized difference vegetation index(NDVI)from Landsat satellite images were integrated for the assessment.Furthermore,we used a digital elevation model(DEM)from ASTER satellite image with 30-m resolution.Aspect and slope maps were derived from this DEM.The soil moisture index(SMI)was obtained using spectral information from Landsat satellite data.We used regression analysis to develop the model.The model shows how SMI from satellite depends on LST,NDVI,DEM,Slope,and Aspect in the agricultural area.The results of the model were correlated with the ground SM data in Tuv province.The results indicate that there is a good agreement between output SM and SM of ground truth for agricultural area.Further research is focused on moisture mapping for different natural zones in Mongolia.The innovative part of this research is to estimate SM using drivers which are vegetation,land surface temperature,elevation,aspect,and slope in the forested steppe area.This integrative methodology can be applied for different regions with forest and desert steppe zones. | Enkhjargal Natsagdorj Tsolmon Renchin Martin Kappas Batchuluun Tseveen Chimgee Dari Oyunbileg Tsend Ulam-Orgikh Duger | 2017 | Geo-Spatial Information Science2017,20,1: | 2 |
| 2 | Transcriptome sequencing analysis for the identification of stable lncRNAs associated with bovine Staphylococcus aureus mastitis显示文摘Background:Staphylococcus aureus(S.aureus)mastitis is one of the most difficult diseases to treat in lactating dairy cows worldwide.S.aureus with different lineages leads to different host immune responses.Long non-coding RNAs(lncRNAs)are reported to be widely involved in the progress of inflammation.However,no research has identified stable lncRNAs among different S.aureus strain infections.In addition,folic acid(FA)can effectively reduce inflammation,and whether the inflammatory response caused by S.aureus can be reduced by FA remains to be explored.Methods:lncRNA transcripts were identified from Holstein mammary gland tissues infected with different concentrations of S.aureus(in vivo)and mammary alveolar cells(Mac-T cells,in vitro)challenged with different S.aureus strains.Differentially expressed(DE)lncRNAs were evaluated,and stable DE lncRNAs were identified in vivo and in vitro.On the basis of the gene sequence conservation and function conservation across species,key lncRNAs with the function of potentially immune regulation were retained for further analysis.The function of FA on inflammation induced by S.aureus challenge was also investigated.Then,the association analysis between these keys lncRNA transcripts and hematological parameters(HPs)was carried out.Lastly,the knockdown and overexpression of the important lncRNA were performed to validate the gene function on the regulation of cell immune response.Results:Linear regression analysis showed a significant correlation between the expression levels of lncRNA shared by mammary tissue and Mac-T cells(P<0.001,R^(2)=0.3517).lncRNAs PRANCR and TNK2-AS1 could be regarded as stable markers associated with bovine S.aureus mastitis.Several HPs could be influenced by SNPs around lncRNAs PRANCR and TNK2-AS1.The results of gene function validation showed PRANCR regulates the mRNA expression of SELPLG and ITGB2 within the S.aureus infection pathway and the Mac-T cells apoptosis.In addition,FA regulated the expression change of DE lncRNA involved in toxin metabolism and inflammation to fight against S.aureus infection.Conclusions:The remarkable association between SNPs around these two lncRNAs and partial HP indicates the potentially important role of PRANCR and TNK2-AS1 in immune regulation.Stable DE lncRNAs PRANCR and TNK2-AS1 can be regarded as potential targets for the prevention of bovine S.aureus mastitis.FA supplementation can reduce the negative effect of S.aureus challenge by regulating the expression of lncRNAs. | Siyuan Mi Yongjie Tang Gerile Dari Yuanjun Shi Jinning Zhang Hailiang Zhang Xueqin Liu Yibing Liu Usman Tahir Ying Yu | 2022 | Journal of Animal Science and Biotechnology2022,13,2: | 2 |
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