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| 1 | Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis显示文摘AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP.METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE Ⅱ score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d1,3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team.RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications;the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli.CONCLUSION: Our study confirmed thatunlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP. | Callum B Pearce Vitaly Zinkevich Iwona Beech Viera Funjika Ana Garcia Ruiz Afraa Aladawi Hamish D Duncan | 2005 | World Journal of Gastroenterology2005，11，45： | 11 |
| 2 | 显示文摘 | Duncan C P Masri B | 1993 | J Bone Joint Surg (Br)1993，75，： | 1 |
| 3 | Constitutive behavior of geosynthetic interface显示文摘 | ESTERHUIZEN J J B FLIZ G M DUNCAN J M | 2001 | Journal of Geotechnical & Geoenvironmental Engineering2001，127，10： | 1 |
| 4 | Ureeolariid eiliates, including three new species, from cultured Philippine fishes显示文摘 | Duncan B L | 1977 | Trans A- mer Micros Soc1977，96，1： | 1 |
| 5 | Theileria annulata sporozoite surface antigen expressed in Escherichia coli elicits neutralizing antibody显示文摘 | Susanna W Andrew T Duncan B | 1989 | PNAS1989，86，： | 1 |
| 6 | Obesity and gestationalweight gain:cesarean delivery and labor complications显示文摘 | Seligman L C Duncan B B Branchtein L | 2006 | Rev Saude Publica2006，40，3： | 1 |
| 7 | Effect of intra- gastric pH on mucosal protective action of sucralfate 显示文摘 | DANESH B J DUNCAN A RUSSELL R I | 1988 | Gut1988，29，10： | 1 |
| 8 | Gamma interferon is the lymphokine and beta interferon the monokine responsible for inhibition of fibroblast collagen production and late but not early fibroblaat proliferation 显示文摘 | Duncan MR Berman B | 1985 | J Exp Med1985，162，2： | 1 |
| 9 | Multiple range and multiple F tests显示文摘 | Duncan D B | 1955 | Biometrics1955，11，： | 1 |
| 10 | The association of dyslipoproteinemia with symptoms and sigtls of peripheral arterial disease:the Lipid Research Clinics Program Prevalence Study显示文摘 | Pomrehn P Duncan B Weissfeld L | 1986 | Circulation1986，73，12： | 1 |
| 11 | Fitting strength criteria to intact rock显示文摘 | Carter B J Duncan S E J Lajtai E Z | 1991 | Geotechnical and Geological Engineering1991，9，1： | 1 |
| 12 | Aprospective study of dietaryintakeandacquiredimmunedeficiencysyndromeinHIV - seropositiveho -mosexualmen显示文摘 | Abrams B Duncan D Hertz - Picciottal | 1993 | JAcquir DeficSyndr1993，6，： | 1 |
| 13 | Educational psychology-some general aspects of learning and teaching显示文摘 | Spence R B Duncan R | 1931 | Psychological Bulletin1931，，28： | 1 |
| 14 | The structure of some simple molecule with lone pair elections显示文摘 | Pople J A | 1953 | Trans Faraday SOC1953，49，： | 1 |
| 15 | Characteristic of organizational environments and perceived environment uncertainty显示文摘 | | 1972 | Administrative Science Quarterly1972，17，3： | 1 |
| 16 | Association between alcoholic and caffeinated beverages and premenstrual syndrome显示文摘 | Caan B Duncan D Hiatt R | 1993 | J Reprod Med1993，38，8： | 1 |
| 17 | Low grade systemic inflammation and the development of type 2 diabetes:The atherosclerosis risk in commmunities study显示文摘 | Duncan B Schmidt M I Pankow J S | 2003 | Diabetes2003，52，7： | 1 |
| 18 | Adult brain ratains the potential to gernerat oligodendroglial progenitors with extensive myelination capacity显示文摘 | Zhang S C Ge B Duncan I D | 1999 | Neurobiology1999，96，7： | 1 |
| 19 | Multiple range and multiple F tests 显示文摘 | Duncan D B | 1955 | Biometrics1955，11，1： | 1 |
| 20 | Management of adult stroke re- habilitation care: a ellnieal practice guideline 显示文摘 | Duncan PW Zorowitz R Bates B | 2005 | Stroke2005，36，： | 1 |
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