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| 1 | Identification of specific genes and pathways involved in NSAIDs-induced apoptosis of human colon cancer cells显示文摘AIM: To study whether indomethacin (IND), a nonselective cyclooxygenase (COX) inhibitor or NS-398 (NS), a COX-2-selective inhibitor, induces apoptosis in human colon cancer cells and which apoptosis-related genes and pathways are involved. METHODS: Human colon cancer Caco-2 cells were treated with either: placebo, IND (0.05-0.5 mmol/L) or NS (0.01-0.2 mmol/L) for 1, 5 and 18 h. We then studied: (1) Cell death by the TUNEL method, (2) mRNA expression of 96 apoptosis-related genes using DNA microarray, (3) expression of selected apoptosis related proteins by Western blotting. RESULTS: Both IND and NS induced apoptosis in 30%-50% of Caco-2 cells in a dose dependent manner. IND (0.1 mmol/L for 1 h) significantly up-regulated pro-apoptotic genes in four families: (1) TNF receptor and ligand, (2) Caspase, (3) Bcl-2 and (4) Caspase recruiting domain. NS treatment up-regulated similar pro-apoptotic genes as IND. In addition, IND also down-regulated anti-apoptotic genes of the IAP family. CONCLUSION: (1) Both non-selective and COX-2-selective NSAIDs induce apoptosis in colon cancer cells in a dose dependent manner. (2) Both NSAIDs induce apoptosis by activating two main apoptotic pathways: the death receptor pathway (involving TNF-R) and the mitochondrial pathway. (3) IND induces apoptosis by up-regulating pro-apoptotic genes and down-regulating anti-apoptotic genes, while NS only up-regulates pro-apoptotic genes. (4) Induction of apoptosis in coloncancer cells by NSAIDs may explain in part, their inhibitory action on colon cancer growth. | Richard H Huang Jianyuan Chai Andrzej S Tarnawski | 2006 | World Journal of Gastroenterology2006，12，40： | 11 |
| 2 | 胡萝卜软腐果胶杆菌巴西亚种(Pectobacterium carotovorum subsp.brasiliense)引起的黄瓜细菌性软腐病在中国发生显示文摘2014—2015年,在中国山东、山西、河北、河南、辽宁等省的黄瓜茎叶上发生了毁灭性的细菌性软腐病,造成了黄瓜产业严重的经济损失。黄瓜叶片、茎、叶柄和果实表面出现流胶;茎基部呈深褐色进而出现湿腐。受侵染的黄瓜叶边缘出现黄斑和湿腐症状,并逐渐扩散至叶片中心。从受侵染组织中分离出45个细菌菌株,在形态学特性鉴定、生理学、生物化学和16Sr RNA基因序列分析的基础上,将病原体鉴定为胡萝卜软腐果胶杆菌(Pectobacterium carotovorum)。多位点序列分析(MLSA)证实,分离菌株是胡萝卜软腐果胶杆菌巴西亚种[Pectobacterium carotovorum subsp.brasiliensis(Pcb)],病原菌归入进化枝II。重新分离获得与原始菌株相同的菌株,证实了分离菌株的致病力。寄主范围测试表明,该菌株具有广泛的寄主范围。目前为止,这是对Pcb引起的黄瓜软腐病——这一对黄瓜生产具有重要经济影响的病害在中国乃至全球范围内的首次报道。 | 冯志琴(译) meng x l chai a l shi y x xie x w ma z h li b j | 2017 | 中国植保导刊2017，37，7： | 10 |
| 3 | A theoretical analysis on self-organized formation of microbial biofilms 显示文摘 | Chen L M Chai L H | 2006 | Physica A: Statistical Mechanics and Its Applications2006，370，2： | 1 |
| 4 | Two-dimensional modeling of compressive failure in delaminated laminates 显示文摘 | Chai H Babcock C D | 1985 | Journal of Composite Materials1985，19，1： | 1 |
| 5 | Effects of cyclophilin A on cell proliferation and gene expressions in human vascular smooth muscle cells and endothelial cells显示文摘 | Yang H Li M Chai H | 2005 | J Surg Res2005，123，2： | 1 |
| 6 | A relative difference quotient algorithm for discrete optimization显示文摘 | Chai S Sun H C | 1996 | Struc Optim1996，12，1： | 1 |
| 7 | The post-buckling response of a bi-laterally constrained column 显示文摘 | Chai H | 1998 | Journal of the Mechanics and Physics of Solids1998，46，7： | 1 |
| 8 | Research on environmental-friendly anodizing process for magnesium alloys显示文摘 | LUO S L DAI L ZHOU H H CHAI L Y KUANG Y F | 2005 | Journal of Hunan University (Natural Sciences)2005，32，3： | 1 |
| 9 | Intelligent deeoupling control of power plant main steam pressure and powe't output 显示文摘 | Liu H B Li S Y Chai T Y | 2003 | International Journal of Electrical Power and Energy System2003，25，10： | 1 |
| 10 | Transesterification of veg- etable oil to biodiesel using a heteropolyacid solid catalyst 显示文摘 | Chai F Cao F H Zhai F Y | 2007 | Adv Synth Catal2007，349，7： | 1 |
| 11 | Prospects for tooth regeneration in the 21st century: a perspective 显示文摘 | Chai Y Slavkin H C | 2003 | Microsc Res Tech2003，60，5： | 1 |
| 12 | Effect of near-fauh vertical ground motions on seismic response of highway overcrossings 显示文摘 | Kunnath S K Erduran E Chai Y H | 2008 | Bridge Engineering2008，13，3： | 1 |
| 13 | The effects of shopping orientations, online trust and prior online pur- chase experience toward customers' online purchase inten- tion显示文摘 | Ling K C Chai L T Piew T H | 2010 | International Business Research2010，3，3： | 1 |
| 14 | Ultimate shear strength of long web panels 显示文摘 | Sung C Lee Doo S Lee Chai H Yoo | 2008 | Journal of Constructional Steel Research2008，64，： | 1 |
| 15 | Effect of hybrid circle reser- voir injected with wavelet-neurons on performance of echo state network 显示文摘 | ] H Cui C Feng Y Chai | 2014 | Neural Networks2014，57，： | 1 |
| 16 | Effect of Near-Fault Vertical Ground Motions on Seismic Re sponse of Highway Overcrossings 显示文摘 | Kunnath S K Erduran E Chai Y H | 2008 | Journal of Bridge Engineering2008，13，3： | 1 |
| 17 | Incorporating low-cycle fatigue model into duration-dependent inelastic design spectra 显示文摘 | Chai Y H | 2005 | Earthquake Engineering and Structural Dynamics2005，34，1： | 1 |
| 18 | A new statistical dynamic analysis on vegetation patterns in land ecosystems显示文摘 | Feng Q Y Chai L H | 2008 | Physica A: Statistical Mechanics and Its Applications2008，387，14： | 1 |
| 19 | Roles of neuropilins in neuronal development, angiogenesis, and cancers显示文摘 | Chen C Li M Chai H | 2005 | World J SurF2005，29，3： | 1 |
| 20 | Source identification of airborne Escherichia coli of swine house surroundings using ERIC-PCR and REP-PCR 显示文摘 | Duan H Chai T Liu J | 2009 | Environ Res2009，109，： | 1 |
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