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| 1 | Hypoxia/oxidative stress alters the pharmacokinetics of CPU86017-RS through mitochondrial dysfunction and NADPH oxidase activation显示文摘 | Jie GAO Xuan-sheng DING Yu-mao ZHANG De-zai DAI Mei LIU Can ZHANG Yin DA | 2013 | Acta Pharmacologica Sinica2013，34，12： | 4 |
| 2 | Detection of human boca virus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in southern brazil显示文摘 | Pilger DA Can tarelli VV Amantea SL | 2011 | Mem Inst Oswaldo Cruz2011，106，1： | 1 |
| 3 | Possible regulation of feeding behavior in cockroach nymphs by the neurotransmitter octopamine显示文摘 | Cohen RW Mahoney DA Can HD | 2002 | J Insect Behav2002，15，1： | 1 |
| 4 | Thyroid incidentalomans:prevalence by palpation and ultrasonography显示文摘 | Ezzat S Sarti DA Can DR | 1994 | Arch Intern Med1994，154，： | 1 |
| 5 | Phase I1 study of daily sunitinib in FDG-PET-positive, iodine-refractory differentiated thyroid cancer and metastatic medullary carcinoma of the thyroid with functional imaging correlation显示文摘 | Can' LL Mankoff DA Goulart BH | 2010 | Clin Cancer Res2010，16，21： | 1 |
| 6 | Phase II study of daily sunitinib in FDG-PET-positive, iodine-refractory differentiated thyroid cancer and metastatic medullary carcinoma of the thyroid with functional imaging cor- relation显示文摘 | Can' LL Mankoff DA Goulart BH | 2010 | Clin Cancer Res2010，6，21： | 1 |
| 7 | Prevalence of celiac disease in an urban area of Brazil with predominantly European ancestry显示文摘AIM: To determine the prevalence of celiac disease in a group of volunteer blood donors at a blood bank in the city of Curitiba, Brazil through detection of the serum marker immunoglobulin A (IgA) antitransglutaminase an-tibody.METHODS: Blood samples collected from 2086 healthy subjects at the Paraná State Center for Hematology and Hemotherapy in Curitiba were submitted to ELISA testing for the IgA antitransglutaminase antibody. Positive samples received IgA antiendomysium antibody test through indirect immunofluorescence using human umbilical cord as substrate. Subsequently, patients who were positive on both tests underwent small bowel (distal duodenum) biopsy.RESULTS: Six subjects, four males and two females, tested positive for the two serum markers. Five of the six were submitted to intestinal biopsy (one declined the procedure). Biopsy results revealed changes in the distal duodenum mucosa (three classified as Marsh Ⅲb lesions and two as Marsh Ⅱ lesions). Most donors diagnosed having celiac disease presented multiple symptoms (gastrointestinal tract complaints). One donor reported having a family history of celiac disease (in a niece).CONCLUSION: Among apparently healthy blood donors,the prevalence of biopsy-confirmed celiac disease was approximately 1:417, similar to that seen in European countries. | Maria Angélica G Pereira Carmen L Ortiz-Agostinho Iêda Nishitokukado Maria N Sato Adérson OMC Damio Marília L Alencar Clarice P Abrantes-Lemos Eduardo LR Canado Thales de Brito Sérgio O Ioshii Sandra BM Valarini Aytan M Sipahi | 2006 | World Journal of Gastroenterology2006，12，40： | 1 |
| 8 | Detection of human boca virus and human metapneumovirus by real-time PCR from pa- tients with respiratory symptoms in southern brazil 显示文摘 | Pilger DA Can tarelli W Amantea SL | 2011 | Mere Inst Oswaldo Cruz2011，106，1： | 1 |
| 9 | Bumetanide alleviates epileptogenic and neurotoxic effects of sevoflurane in neonatal rat brain显示文摘 | Edwards DA Shah HP Can W | 2010 | Anesthesiology2010，112，3： | 1 |
| 10 | Functional analysis of flavanone 3-hydroxylase(F3H)from Dendrobium officinale,which confers abiotic stress tolerance显示文摘Flavonoids are important bioactive components in Dendrobium officinale,a medicinal orchid.They are involved in many biological activities,including protecting plants against biotic and abiotic stresses.Research on the key genes related to flavonoid biosynthesis in D.officinale is limited.In this study,one of the key flavonoid biosynthesis genes,flavanone 3-hydroxylase(F3H),was characterized from D.officinale.The open reading frame of DoF3H was 1134 bp long and it encoded a 377-amino acid protein.The DoF3H protein showed considerably high homology with F3H proteins from other plant species and shared a common evolutionary ancestor with other F3Hs.DoF3H transcripts were detected in different organs of adult plants and mainly accumulated in flowers,followed by roots,stems and leaves,a pattern that was similar to the content of flavonoids.Recombinant DoF3H protein,which was localized in the cytosol,could convert naringenin to dihydrokaempferol.The mRNA levels of DoF3H were significantly induced by salt and cold stresses.Furthermore,the heterologous expression of DoF3H in Escherichia coli conferred it higher tolerance to salt and cold stresses.These results provide insight into the molecular function of DoF3H in the biosynthesis of flavonoids,and provide a new application for improvement of abiotic tolerance in D.officinale. | Can Si Wei Dong Jaime A.Teixeira da Silva Chunmei He Zhenming Yu Mingze Zhang Lei Huang Conghui Zhao Danqi Zeng Chuanmao Li Shengxiang Qiu Jun Duan | 2023 | Horticultural Plant Journal2023，9，2： | 1 |
| 11 | Alphal-antitrypsin deficiency-a model for conformational diseases 显示文摘 | Can'ell RW Lomas DA | 2002 | New Engl J Med2002，346，1： | 1 |
| 12 | Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality显示文摘Background:In vitro production of bovine embryos is a well-established technology,but the in vitro culture(IVC)system still warrants improvements,especially regarding embryo quality.This study aimed to evaluate the effect of extracellular vesicles(EVs)isolated from oviductal(OF)and uterine fluid(UF)in sequential IVC on the development and quality of bovine embryos.Zygotes were cultured in SOF supplemented with either BSA or EVs-depleted fetal calf serum(dFCS)in the presence(BSA-EV and dFCS-EV)or absence of EVs from OF(D1 to D4)and UF(D5 to D8),mimicking in vivo conditions.EVs from oviducts(early luteal phase)and uterine horns(mid-luteal phase)from slaughtered heifers were isolated by size exclusion chromatography.Blastocyst rate was recorded on days 7-8 and their quality was assessed based on lipid contents,mitochondrial activity and total cell numbers,as well as survival rate after vitrification.Relative mRNA abundance for lipid metabolism-related transcripts and levels of phosphorylated hormonesensitive lipase(pHSL)proteins were also determined.Additionally,the expression levels of 383 miRNA in OF-and UF-EVs were assessed by qRT-PCR.Results:Blastocyst yield was lower(P<0.05)in BSA treatments compared with dFCS treatments.Survival rates after vitrification/warming were improved in dFCS-EVs(P<0.05).EVs increased(P<0.05)blastocysts total cell number in dFCS-EV and BSA-EV compared with respective controls(dFCS and BSA),while lipid content was decreased in dFCSEV(P<0.05)and mitochondrial activity did not change(P>0.05).Lipid metabolism transcripts were affected by EVs and showed interaction with type of protein source in medium(PPARGC1B,LDLR,CD36,FASN and PNPLA2,P<0.05).Levels of pHSL were lower in dFCS(P<0.05).Twenty miRNA were differentially expressed between OF-and UF-EVs and only bta-miR-148b was increased in OF-EVs(P<0.05).Conclusions:Mimicking physiological conditions using EVs from OF and UF in sequential IVC does not affect embryo development but improves blastocyst quality regarding survival rate after vitrification/warming,total cell number,lipid content,and relative changes in expression of lipid metabolism transcripts and lipase activation.Finally,EVs miRNA contents may contribute to the observed effects. | Cláudia Lima Verde Leal Karina Canón-Beltrán Yulia N.Cajas Meriem Hamdi Aracelli Yaryes María Gemma Millán de la Blanca Paula Beltrán-Brena Rosane Mazzarella Juliano Coelho da Silveira Alfonso Gutiérrez-Adán Encina M González Dimitrios Rizos | 2023 | Journal of Animal Science and Biotechnology2023，14，1： | 0 |
| 13 | Immunogenicity of Whole Mycobacterium intracellulare Proteins and Fingding on the Cross-Reactive Proteins between M.intracellulare and M.tuberculosis显示文摘Objectives To evaluate the immunogenicity of Mycobacterium intracellulare proteins and determine the cross-reactive proteins between M.intracellulare and M.tuberculosis.Methods Protein extracts from M.intracellulare were used to immunize BALB/c mice.The antigens were evaluated using cellular and humoral immunoassays.The common genes between M.intracellular and M.tuberculosis were identified using genome-wide comparative analysis,and cross-reactive proteins were screened using immunoproteome microarrays.Results Immunization with M.intracellulare proteins induced significantly higher levels of the cytokines interferon-γ(IFN-γ),interleukin-2(IL-2),interleukin-12(IL-12),interleukin-6(IL-6)and immunoglobulins IgG,IgG1,IgM,and IgG2a in mouse serum.Bone marrow-derived macrophages isolated from mice immunized with M.intracellulare antigens displayed significantly lower bacillary loads than those isolated from mice immunized with adjuvants.Whole-genome sequence analysis revealed 396 common genes between M.intracellulare and M.tuberculosis.Microchip hybridization with M.tuberculosis proteins revealed the presence of 478 proteins in the serum of mice immunized with M.intracellulare protein extracts.Sixty common antigens were found using both microchip and genomic comparative analyses.Conclusion This is the advanced study to investigate the immunogenicity of M.intracellulare proteins and the cross-reactive proteins between M.intracellulare and M.tuberculosis.The results revealed the presence of a number of cross-reactive proteins between M.intracellulare and M.tuberculosis.Therefore,this study provides a new way of identifying immunogenic proteins for use in tuberculosis vaccines against both M.intracellulare and M.tuberculosis in future. | XIAO Shi Qi XU Da DUAN Hong Yang FAN Xue Ting LI Gui Lian ZHANG Wen LI Ma Chao HAN Na LI Xin Yao LI Na ZHAO Li lan ZHAO Xiu Qin WAN Kang Lin LIU Hai Can FENG Wen Hai | 2021 | Biomedical and Environmental Sciences2021，34，7： | 0 |
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