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| 1 | Distribution of the P2X2 receptor and chemical coding in ileal enteric neurons of obese male mice(ob/ob)显示文摘AIM:To investigate the colocalization,density and profile of neuronal areas of enteric neurons in the ileum of male obese mice.METHODS:The small intestinal samples of male mice in an obese group(OG)(C57BL/6J ob/ob)and a control group(CG)(+/+)were used.The tissues were analyzed using a double immunostaining technique for immunoreactivity(ir)of the P2X2 receptor,nitric oxide synthase(NOS),choline acetyl transferase(ChAT)and calretinin(Calr).Also,we investigated the density and profile of neuronal areas of the NOS-,ChAT-and Calrir neurons in the myenteric plexus.Myenteric neurons were labeled using an NADH-diaphorase histochemical staining method.RESULTS:The analysis demonstrated that the P2X2receptor was expressed in the cytoplasm and in the nuclear and cytoplasmic membranes only in the CG.Neuronal density values(neuron/cm2)decreased 31%(CG:6579±837;OG:4556±407)and 16.5%(CG:7796±528;OG:6513±610)in the NOS-ir and calretininir neurons in the OG,respectively(P<0.05).Density of ChAT-ir(CG:6200±310;OG:8125±749)neurons significantly increased 31%in the OG(P<0.05).Neuron size studies demonstrated that NOS,ChAT,and Calr-ir neurons did not differ significantly between the CG and OG groups.The examination of NADH-diaphorase-positive myenteric neurons revealed an overall similarity between the OG and CG.CONCLUSION:Obesity may exert its effects by promoting a decrease in P2X2 receptor expression and modifications in the density of the NOS-ir,ChAT-ir and CalR-ir myenteric neurons. | Márcia Sanae Mizuno Amanda Rabello Crisma Primavera Borelli Bárbara Tavares Schfer Mariana Póvoa Silveira Patricia Castelucci | 2014 | World Journal of Gastroenterology2014，20，38： | 3 |
| 2 | Expression of the P2X_2 receptor in different classes of ileum myenteric neurons in the female obese ob/ob mouse显示文摘AIM:To examine whether the ob/ob mouse model of obesity is accompanied by enteric nervous system ab-normalities such as altered motility METHODS:The study examined the distribution of the P2X 2 receptor (P2X 2 R) in myenteric neurons of female ob/ob mice. Specifically, we used immunohistochemistry to analyze the co-expression of the P2X 2 R with neuronal nitric oxide synthase (nNOS), choline acetyltrans-ferase (ChAT), and calretinin (CalR) in neurons of the small intestine myenteric plexus in ob/ob and control female mice In these sections, we used scanning confocal microscopy to analyze the co-localization of these markers as well as the neuronal density (cm 2 ) and area profile (μm2) of P2X 2 R-positive neurons In addition, enteric neurons were labeled using the nicotinamide adenine dinucleotide (NA H) diaphorase method and analyzed with light microscopy as an alternate means by which to analyze neuronal density and areaRESULTS:In the present study, we observed a 29 6% increase in the body weight of the ob/ob animals (OG) compared to the control group (CG) In addition, the average small intestine area was increased by approxi-mately 29 6% in the OG compared to the CG Immu-noreactivity (IR) for the P2X 2 R, nNOS, ChAT and CalR was detectable in the myenteric plexus, as well as in the smooth muscle, in both groups This IR appeared to be mainly cytoplasmic and was also associated with the cell membrane of the myenteric plexus neurons, where it outlined the neuronal cell bodies and their processes P2X 2 R-IR was observed to co-localize 100% with that for nNOS, ChAT and CalR in neurons of both groups In the ob/ob group, however, we observed that the neuronal density (neuron/cm 2 ) of P2X 2 R-IR cells was in-creased by 62% compared to CG, while that of NOS-IR and ChAT-IR neurons was reduced by 49% and 57%, respectively, compared to control mice The neuronal density of CalR-IR neurons was not different between the groups Morphometric studies further demonstrated that the cell body profile area (μm2) of nNOS-IR, ChAT-IR and CalR-IR neurons was increased by 34%, 20% and 55%, respectively, in the OG compared to controls Staining for NA H diaphorase activity is widely used to detect alterations in the enteric nervous system; however, our qualitative examination of NA H-diaphorase positive neurons in the myenteric ganglia revealed an overall similarity between the two groups CONCLUSION:We demonstrate increases in P2X2R expression and alterations in nNOS, ChAT and CalR IR in ileal myenteric neurons of female ob/ob mice compared to wild-type controls. | Márcia Sanae Mizuno Amanda Rabello Crisma Primavera Borelli Patricia Castelucci | 2012 | World Journal of Gastroenterology2012，18，34： | 2 |
| 3 | Distribution of Fos immunoreactivity in the rat brain after freezing or escape elicited by inhibition of glutamic acid decarboxylase or antagonism of GABA-A receptors in the inferior collieulus显示文摘 | Borelli K G Ferreira-Netto C Brandiao M L | 2006 | Behavioural Brain Research2006，170，1： | 1 |
| 4 | Specific sHLA in healthy donors and donor-specific sHLA in renal transplant patients 显示文摘 | Borelli SD Ferreira E Oliveira AM | 1999 | Hum Immunol1999，60，5： | 1 |
| 5 | Candida albicanssecreted aspartic proteinases modify the epithelial cytokine response in an in vitro model of vaginal candidiasis显示文摘 | Schaller M Korting HC Borelli C | 2005 | Infect Immun2005，73，5： | 1 |
| 6 | Valaciclovir versus acyclo vir in patient initiated treatment of recurrent genital herpes:a randomised,do uble blind clinical trial显示文摘 | Bodsworth NJ Crooks RJ Borelli S | 1997 | Genitourin Med1997，73，2： | 1 |
| 7 | Modulation of innate and adaptive immunity by lactoferrin in human immuno-defi-ciency virus(HIV)-infected,antiretroviral therapy nate children显示文摘 | ZUCCOTTI G V VIGANO A BORELLI M | 2007 | Int J Antimicrob Agents2007，29，3： | 1 |
| 8 | Valaciclovir versus aciclovir in patient initiated treatment of recurrent genital herpes: a randomised, double blind clinical trial 显示文摘 | Bodsworth N J Crooks R J Borelli S | 1997 | Genitourin Med1997，73，： | 1 |
| 9 | Optically induced anisotropy in photo chromic glasses显示文摘 | BORELLI N F CHODAK J B HARES G B | 1979 | J Appl Phys1979，50，9： | 1 |
| 10 | Safety and efficacy of sertaconazole nitrate cream 2% in the treatment of tinea pedis interdigitalis : a subgroup analysis 显示文摘 | Borelli C Korting HC BSdeker RH | 2010 | Curls2010，85，2： | 1 |
| 11 | Islet a- daptive changes to fructose-induced insulin resistance: beta-cell mass, glucokinase, glucose metabolism, and insulin secretion 显示文摘 | MAIZTEHUI B BORELLI MI RASCHIA MA | 2009 | J Endocrinol2009，200，2： | 1 |
| 12 | Hydrolytic enzymes as virulence factors of Candida albicans显示文摘 | SCHALLER M BORELLI C KORTING HC | 2005 | Mycoses2005，48，6： | 1 |
| 13 | Application of para-nitrophenol (pNP) enzyme assays in degraded tropical soils显示文摘 | Verchot LV Borelli T | 2005 | Soil Biology & Biochemistry2005，37，： | 1 |
| 14 | Hydrolytic enzymes as viru- lence factors of Candida albicans 显示文摘 | Schaller Borelli M Korting C et at | 2005 | Mycoses2005，48，6： | 1 |
| 15 | Candida albicaas-secreted aspartic proteinases modify the epithelial cytokine response in an in vitro model of vaginal candidiasis 显示文摘 | Schaller M Korting HC Borelli C | 2005 | Infect Immun2005，73，5： | 1 |
| 16 | Comparative study of 2% sertaconazole solution and cream formulations in patients with tinea corporis, tinea pedis interdigitalis, or a corresponding candidosis 显示文摘 | Borelli C Klovekorn G Ernst TM | 2007 | Am J Clin Dermatol2007，8，6： | 1 |
| 17 | X-ray structures of Sap1 andSap5:structural comparison of the secreted aspartic proteinasesfrom Candida albicans显示文摘 | Borelli C Ruge E Lee JH | 2008 | Proteins2008，72，4： | 1 |
| 18 | Application of para-nitrophenol (pNP) enzyme assays in degraded tropical soils显示文摘 | VERCHOT L V BORELLI T | 2005 | Soil Biology and Biochemis- try2005，37，4： | 1 |
| 19 | Islet neogenesis: an apparent key component of long-term pancreas adaptation to increased insulin demand显示文摘 | Del Zotto H Borelli MI Flores L | 2004 | J Endocrinol2004，183，2： | 1 |
| 20 | Quantitation of HER-2/neu and c-myc gene amplification in breast carcinoma using fluorescence in situ hybridization 显示文摘 | Persons DL Borelli KA Hsu PH | 1997 | Mod Pathol1997，10，7： | 1 |
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