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| 1 | Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)显示文摘Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypo-cotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods. | RAO Abdul Qayyum HUSSAIN S. Sarfraz SHAHZAD M. Saqib BOKHARI S. Yassir Abbas RAZA M. Hashim RAKHA Allah MAJEED A. SHAHID A. Ali SALEEM Zafar HUSNAIN Tayyab RIAZUDDIN S. | 2006 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2006，7，4： | 9 |
| 2 | Viral respiratory infections among Hajj pilgrims in 2013显示文摘Middle East respiratory syndrome coronavirus(MERS-Co V) has emerged in the Arabian Gulf region, with its epicentre in Saudi Arabia, the host of the ‘Hajj' which is the world's the largest mass gathering. Transmission of MERS-Co V at such an event could lead to its rapid worldwide dissemination. Therefore, we studied the frequency of viruses causing influenza-like illnesses(ILI) among participants in a randomised controlled trial at the Hajj 2013. We recruited 1038 pilgrims from Saudi Arabia, Australia and Qatar during the first day of Hajj and followed them closely for four days. A nasal swab was collected from each pilgrim who developed ILI. Respiratory viruses were detected using multiplex RT-PCR. ILI occurred in 112/1038(11%) pilgrims. Their mean age was 35 years, 49(44%) were male and 35(31%) had received the influenza vaccine pre-Hajj. Forty two(38%) pilgrims had laboratory-confirmed viral infections; 28(25%) rhinovirus, 5(4%) influenza A, 2(2%) adenovirus, 2(2%) human coronavirus OC43/229 E, 2(2%) parainfluenza virus 3, 1(1%) parainfluenza virus 1, and 2(2%) dual infections. No MERS-Co V was detected in any sample. Rhinovirus was the commonest cause of ILI among Hajj pilgrims in 2013. Infection control and appropriate vaccination are necessary to prevent transmission of respiratory viruses at Hajj and other mass gatherings. | Osamah Barasheed Harunor Rashid Mohammad Alfelali Mohamed Tashani Mohammad Azeem Hamid Bokhary Nadeen Kalantan Jamil Samkari Leon Heron Jen Kok Janette Taylor Haitham El Bashir Ziad A.Memish Elizabeth Haworth Edward C.Holmes Dominic E Dwyer Atif Asghar Robert Booy | 2014 | Virologica Sinica2014，29，6： | 3 |
| 3 | Antibiotic susceptibility profiling and virulence potential of Campylobacter jejuni isolates from different sources in Pakistan显示文摘Objective:To determine antibiotic resistance patterns and virulence potential of Campylobacter jejuni(C.jejuni) isolates from clinical human diarrheal infections,cattle and healthy broilers.Methods:Antibiotic sensitivity patterns of C.jejuni isolates were determined by Kirby Bauer Disc Diffusion assay.These isolates were then subjected to virulence profiling for the detection of map A(membrane-associated protein).cadF(fibronectin binding protein).wlaN(beta-1.3-galaclosyltransferase) and neu AB(sialic acid biosynthesis gene).Further C.jejuni isolates were grouped by random amplification of polymorphic DNA(RAPD) profiling.Results:A total of436 samples from poultry(n=88).cattle(n=216) and humans(n=132) from different locations were collected.Results revealed percentage of C.jejuni isolates were 35.2%(31/88).25.0%(54/216) and 11.3%(15/132) among poultry,cattle and clinical human samples respectively.Antibiotic susceptibility results showed that similar resistance patterns to cephalothin was ie.87.0%,87.1%and 89%among humans,poultry and cattle respectively,followed by sulfamethoxazolc+trimcthoprim 40.0%,38.7%and 31.0%in humans,poultry and cattle and Ampicillin 40%,32%and 20%in humans,poultry and cattle respectively.Beta-lactamase activity was detected in 40.00%humans.20.37%cattle and 32.25%in poultry C.jejuni isolates.CadF and mapA were present in all poultry,cattle and human C jejuni isolates.wlauN was not detected in any isolate and neu AB was found in 9/31(36%) poultry isolates.RAPD profiling results suggested high diversity of C.jejuni isolates.Conclusions:Detection of multidrug resistant C.jejuni strains from poultry and cattle is alarming as they can be potential hazard to humans.Moreover,predominant association of virulence factors,cadF and map A(100%each) in C.jejuni isolates from all sources and neuAB(36%) with poultry isolates suggest the potential source of transmission of diverse types of C.jejuni to humans. | Fariha Masood Siddiqui Muhammad Akram Nighat Nourccn Zobia Noreen Habib Bokhari | 2015 | Asian Pacific Journal of Tropical Medicine2015，8，3： | 2 |
| 4 | Novel Cell Culture Device Enabling Three-Dimensional Cell Growth and hnproved Cell Function 显示文摘 | Bokhari M Camachan R J Cameron N R | 2007 | Biochem Biophys Res Comnmn2007，354，4： | 1 |
| 5 | Time course of appearance and disappearance of human mast cell tryptase in the circulation after anaphylaxis显示文摘 | Schwartz LB Yunginger JW Miller J Bokhari R Dull D | 1989 | J Clin Invest1989，83，5： | 1 |
| 6 | Outcomes of silicone oil removal显示文摘 | Zafar S Bokhari S A Kamil Z | 2013 | J Coll Physicians Surg Pak2013，23，7： | 1 |
| 7 | Imaging features of solitary fibrous tumors显示文摘 | Ginat DT Bokhari A Bhatt S | 2011 | AJR2011，196，： | 1 |
| 8 | Endoscopic trans- sphenoidal pituitary surgery: a single surgeon experience and the learning curve 显示文摘 | Bokhari AR Davies MA Diamond T | 2013 | Br J Neurosurg2013，27，1： | 1 |
| 9 | Outcomes of silicone oil re- moval显示文摘 | Zafar S Bokhari SA Kamil Z | 2013 | J Coil Physicians Surg Pak2013，23，7： | 1 |
| 10 | A partitioning strategy for nonuniform proble ms on multiprocessors 显示文摘 | Berger M J Bokhari S H | 1987 | IEEE Transactions on Computers1987，36，5： | 1 |
| 11 | Clinical practice guideline : endpoints of resuscitation 显示文摘 | Tisherman S A Barie P Bokhari F | 2004 | J Trauma2004，57，4： | 1 |
| 12 | Laparoscopic versus poen repair of incisional/ventral hernia:a meta-analysis显示文摘 | Muhammad SS Syed A Bokhari I | 2009 | Am J Surg2009，197，1： | 1 |
| 13 | Strong association of a renin intronic dimorphism with essential hypertension 显示文摘 | Ahmad U Saleheen D Bokhari A | 2005 | Hypertension Res2005，28，4： | 1 |
| 14 | Recovery of older patients undergoing ambulatory anaesthesia with iso显示文摘 | Mahajan VA Ni Chonghaile M Bokhari SA | 2007 | Eur J Anaesthesiol2007，24，6： | 1 |
| 15 | Incorporating burr type XII testing-efforts into software reliability growth modeling and actual data analysis with applications显示文摘 | BOKHARI M U AHMAD N | 2014 | Journal of Software2014，9，6： | 1 |
| 16 | Positron emission tomography imaging may provide a novel biomarker and understanding of right ventricular dysfunction in patients with idiopathic pulmonary arterial hypertension显示文摘 | Bokhari S Raina A Rosenweig EB | | Circ Cardiovasc Imaging0，，： | 1 |
| 17 | Glycemic changes after vitamin D supplementation in patients with type 1 diabetes mellitus and vitamin D deficiency显示文摘 | Aljabri KS Bokhari SA Khan MJ | 2010 | Ann Saudi Med2010，30，： | 1 |
| 18 | Coupling of Surface Carboxyls of Carboxymethylcellulase with Aniline via Chemical Modification: Extreme Thermostabilization in Aqueous and Water-Miscible Organic Mixtures显示文摘 | Bokhari S A Afzal A J Rashid M H | 2002 | Biotechnol Prog2002，18，： | 1 |
| 19 | Acute septic arthritis in children显示文摘 | Al Saadi MM Al Zami FA Bokhary NA | 2009 | Pediatr Int2009，51，3： | 1 |
| 20 | Current advance in the diagnosis and treatment of renal artery stenosis显示文摘 | Bokhari SW Faxon DP | 2004 | Rev Cardiovasc Med2004，5，4： | 1 |
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