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| 1 | 移植分泌神经营养因子-3的人胚神经干细胞对大鼠脊髓损伤后功能恢复的影响显示文摘目的探索Lentivirus介导神经营养因子(NT-3)转染的人胚神经干细胞(human neural stem cells,hNSCs)对大鼠损伤脊髓治疗机制和治疗的作用机制。方法在体外用Lentivirus构建同时表达绿色荧光蛋白(green fluorescence protein,GFP)和NT-3的hNSCs,将其移植到T10脊髓半横断损伤的大鼠体内,用荧光显微镜和BBB评分观察体内转基因表达和大鼠功能恢复情况,用免疫组化染色检测移植细胞在体内存活分化情况。结果(1)在体外用Lentivirus成功构建了同时表达多基因的人胚基因工程NSC并检测到了稳定的转基因表达;(2)用荧光显微镜可以检测到到hNSC,能在体内长时间存活并表达转基因;(3)BBB评分检测到hNSCs移植组大鼠后肢功能有明显恢复;(4)移植的基因工程hNSC能在体内分化为神经元及星形胶质细胞等功能细胞。结论Lentivirus介导NT-3转染的hNSC能够调整损伤的局部微环境促进损伤脊髓的功能恢复。 | 孙广运 蔡培强 Oudega Martin 王学文 Blits Bas 舒运兵 蔡程 | 2007 | 中华创伤杂志2007，23，2： | 8 |
| 2 | GENETIC ENGINEERING NEURAL STEM CELL MODIFIED BY LENTIVIRUS FOR REPAIR OF SPINAL CORD INJURY IN RATS显示文摘Objective To explore the feasibility for therapy of spinal cord injury (SCI) by genetic engineering neural stem cell (NSC) modified by lentiviral vector. Methods Following the construction of the genetic engineering NSC modified by lentivirus to secrete both neurotrophic factor-3 (NT-3) and green fluorescence protein (GFP), hemisection of spinal cord at the level of T_ 10 was performed in 56 adult Wistar rats that were randomly divided into 4 groups (n=14), namely 3 therapeutic groups and 1 control group. The therapeutic groups were dealed with NSC, genetic engineering NSC, and concentrated lentiviral supernatant which carries both GFP and NT-3, respectively. Then used fluorescence microscope to detect the transgenic expression in vitro and in vivo, migration of the grafted cells in vivo, and used the Basso, Beattie, and Bresnahan (BBB) open-field locomotor test to assess the recovery of function. Results The transplanted cells could survive for long time in vivo and migrate for long distance. The stable transgenic expression could be detected in vivo. The hindlimb function of the injured rats in 3 therapeutic groups, especially those dealed with genetic engineering NSC, improved obviously. Conclusion It is feasible to combine NSC with lentivirus for the repair of SCI. NSC modified by lentivirus to deliver NT-3, acting as a source of neurotrophic factors and function cell in vivo, has the potential to participate in spinal cord repair. | Xun Tang Pei-qiang Cai Yue-qiu Lin Martin Oudega Bas Blits Ling Xu Yun-kang Yang Tian-hua Zhou | 2006 | Chinese Medical Sciences Journal2006，21，2： | 8 |
| 3 | Lentivirus介导分泌神经营养因子-3的基因工程神经干细胞移植治疗脊髓损伤的实验研究显示文摘 | 蔡培强 汤逊 林月秋 Martin Oudega Bas Blits 徐林 阳运康 | 2005 | 中华外科杂志2005，43，16： | 4 |
| 4 | Intercostal nerve implants t ransduced with an adenoviral vector encoding neurotrophin-3 promote regrowth of injured rat corticospinal tract fibers and improve hindlimb function显示文摘 | Blits B Dijkhuizen PA Boer GJ | 2000 | Exp Neurol2000，164，1： | 2 |
| 5 | Lentivirus介导神经营养因子-3促进脊髓损伤大鼠功能恢复显示文摘目的探讨Lentivirus介导分泌神经营养因子-3(NT-3)直接体内转基因治疗大鼠脊髓损伤作用和机制。方法将28只Wistar大鼠在T10水平制成半横断损伤模型,随机分为体内转基因治疗组和损伤对照组,每组14只;用携带NT-3和绿色荧光蛋白(GFP)的Lentivirus对大鼠行直接体内转基因治疗;荧光显微镜观察体内转基因表达,后肢运动功能评分法(BBB评分)检测大鼠后肢功能恢复情况。结果用荧光显微镜检测到损伤脊髓内有较多的持续表达转基因的细胞;BBB评分结果显示,从伤后第4周开始,实验组大鼠后肢功能较对照组明显恢复(P<0.01),至第10周时,实验组和对照组BBB分差增大,达3.3分。结论Lentivirus是一种有效的转基因载体,由Lentivirus介导分泌NT-3的直接体内转基因治疗能够明显促进损伤脊髓的功能恢复。 | 蔡培强 汤逊 林月秋 Oudega Mart 徐林 Blits B 阳运康 M.OUDEGA | 2005 | 中华创伤杂志2005，21，7： | 2 |
| 6 | Transplantation of BDNF-secreting mesenchymal stem cells provides neuroprotection in chronically hypertensive rat eyes显示文摘 | Harper MM Grozdanic SD Blits B Kuehn MH Zamzow D Buss JE | 2011 | Invest Optuhalmol Vis Sci2011，52，7： | 1 |
| 7 | The formation of laser beams with pure azimuthal or radial polarization显示文摘 | Oron R Blit S Davidson N | 2000 | Applied Physics Letters2000，77，： | 1 |
| 8 | Intravitreal injection of adeno-associated viral vectors results in the transduction of different types of retinal neurons in neonatal and adult rats:a comparison with lentiviral vectors显示文摘 | Harvey AR Kamphuis W Eggers R Symons NA Blits B Niclou S et ol | 2002 | Mol Cell Neurosci2002，21，1： | 1 |
| 9 | Intercostal nerve implants transduced with an adenoviral vector encoding neurotrophin-3 promote regrowth of injured rat corticospinal tract fibers and improve hindlimb function显示文摘 | BLITS B DIJKHUIZEN P A BOER G J | 2000 | Exp Neurol2000，164，1： | 1 |
| 10 | Transplantation of BDNF- secreting mesenehymal stem eells provides neuroproteetion in chronically hypertensive rat eyes显示文摘 | Harper MM Grozdanie SD Blits B | 2011 | Invest Ophthalmol Vis Sei2011，52，7： | 1 |
| 11 | Effect of NaCl on lipid content of plasma membranes isolated from root sand cell suspension cultures of the dicot halophyte Kosteletzkya virginica显示文摘 | Blits K C Gallaghet J L | 1990 | Plant Cell Reports1990，9，： | 1 |
| 12 | Adeno-associated viral vectormediated neurotrophin gene transfer in the injured adult rat spinal cord improves hind-limc function显示文摘 | Blits B Oudega M Boer GJ | 2003 | Neurosci2003，118，1： | 1 |
| 13 | Methotrexate nor- malizes up-regulated folate pathway genes in rheumatoid arthritis显示文摘 | Blits M Jansen G Assaraf YG | 2013 | Arthritis Rheum2013，65，11： | 1 |
| 14 | Intercostal nerve implants transduced with an adenoviral vector encoding neurotrophin-3 promote regrowth of injured rat corticospinal tract fibers and improve hindlimb function显示文摘 | Blits B Dijkhuizen PA Boer GJ | 2000 | Exp Neurol2000，164，1： | 1 |
| 15 | Platelet inhibition and endothelial cell adhesion on elastin-like polypeptide surface modified materials显示文摘 | Blit P H McClung W G Brash J L | 2011 | Biomaterials2011，32，25： | 1 |
| 16 | Intercostal nerve implants transduced with andenoviral vector encoding neurotrophin - 3 promote growth of injured rat corticospinal tract fibers and improve hind limb function显示文摘 | Blits B Dijkhuizen PA Boer GJ | 2000 | Exp Neurol2000，164，1： | 1 |
| 17 | Adeno-aasociated viral vector-mediated neurotrophin gene transfer in the injured adult rat spinal cord improves hind~limb function显示文摘 | Blits B Oudega M Boer GJ | 2003 | Neuroscience2003，118，： | 1 |
| 18 | Amelioration of chronic neuropathic pain after partial nerve injury by adenoassociated viral (AAV) vector-mediated over-expression of BDNF in the rat spinal cord 显示文摘 | Eaton MJ Blits B Rultenberg MJ | 2002 | Gene Ther2002，9，20： | 1 |
| 19 | Large tunable delay with low distortion of 10Gbit/s data in a slow light system based on narrow band fiber parametric amplification 显示文摘 | SHUMAKER E WILLINGER A BLIT R | 2006 | OPTICS EXPRESS2006，14，19： | 1 |
| 20 | Intercostal nerve implants transduced with an adenoviral vector encoding neurotrophin-3 promote regrowth of injured rat corticospinal tract fibers and improve hindlimb function显示文摘 | Dijkhuizen P A Boer G J | 2000 | Exp Neurol2000，164，： | 1 |
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