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| 1 | Role of the advanced glycation end products receptor in Crohn's disease inflammation显示文摘AIM:To investigate the level of mucosal expression and the involvement of the receptor for the advanced glycation end products(RAGE)in delayed apoptosis and tumor necrosis factor(TNF)-αproduction in Crohn’s disease(CD).METHODS:Surgical and endoscopic specimens from both inflamed and non-inflamed areas of the ileum and/or colon were collected from 20 and 14 adult CD patients,respectively,and used for the assessment of RAGE expression by means of immunohistochemistry and western blotting analysis.Normal tissues from 21 control subjects were used for comparison.The same polyclonal anti-human RAGE antibody(R and D System)was used in all experimental conditions.RAGE staining was quantized by a score including both the amount of positive cells and intensity of immunoreactivity;cellular pattern was also described.The effects of RAGE blocking on apoptotic rate and TNF-αproduction were investigated on immune cells freshly isolated from CD mucosa and incubated both with and without the muramyl dipeptide used as antigenic stimulus.Statistical analysis was performed via the test for trend,with regression models to account for intra-patient correlations.A 2-sided P<0.05 was considered significant.RESULTS:In inflamed areas,RAGE expression in both the epithelial and lamina propria compartments was higher than control tissues(P=0.001 and 0.021,respectively),and a cluster of positive cells were usually found in proximity of ulcerative lesions.Similar results were obtained in the lamina propria compartment of non-inflamed areas(P=0.025).The pattern of staining was membranous and granular cytosolic at the epithelial level,while in the lamina propria it was diffuse cytosolic.When evaluating the amount of protein expression by immunoblotting,a significant increase of both surface area and band intensity(P<0.0001 for both)was observed in CD inflamed areas compared to control tissue,while in non-inflamed areas a significant increase was found only for band intensity(P<0.005).Moreover,a significantly lower expression in noninflamed areas in comparison with inflamed areas was found for both surface area and band intensity(P<0.0006 for both).Finally,RAGE blocking largely affects both the apoptotic rate of mucosal cells(towards an increase in both non-inflamed and inflamed areas of P<0.001 and<0.0001,respectively)and TNF-αsecretion(towards a decrease in both non-inflamed and inflamed areas of P<0.05 and<0.01,respectively),mainly in the presence of antigenic stimulation.CONCLUSION:RAGE is up-regulated in CD,especially in inflamed areas,and it appears to play a role in the mechanisms involved in chronic inflammation. | Rachele Ciccocioppo Alessandro Vanoli Catherine Klersy Venerina Imbesi Vincenzo Boccaccio Rachele Manca Elena Betti Giuseppina Cristina Cangemi Elena Strada Roberta Besio Antonio Rossi Colomba Falcone Sandro Ardizzone Paolo Fociani Piergiorgio Danelli Gino Roberto Corazza | 2013 | World Journal of Gastroenterology2013，19，45： | 2 |
| 2 | Invasive growth : a MET - driven genetic programme for cancer and stem cells 显示文摘 | Boccaccio C Comoglio PM | 2006 | Nat Rev Cancer2006，6，8： | 1 |
| 3 | A mechano regulation model of fracture repair in vertebral bodies显示文摘 | BOCCACCIO A KELLY D J PAPPALETTERE C | 2011 | J OrthopRes2011，29，3： | 1 |
| 4 | Invasive growth:a MET-driven genetic programme for cancer and stem cells显示文摘 | Boccaccio C Comoglio PM | 2006 | Nat Rev Cancer2006，6，8： | 1 |
| 5 | Induction of epithelial tubules by growth factor HGF depends on the STAT pathway显示文摘 | Boccaccio C Andò M Tamagnone L | | 0，，6664： | 1 |
| 6 | Unusual intracellular and ex- tracellular crystal inclusions in light chain multiple myeloma 显示文摘 | Casciaro S Clavio M Boccaccio P | 1999 | Haematologica1999，84，11： | 1 |
| 7 | Induction of epithelial tubules by growth factor HGF depends on the STAT pathway显示文摘 | Boccaccio C Ando M Tamagnone L | 1998 | Nature1998，391，6664： | 1 |
| 8 | Incoming human cytomegalovirus pp65 (UL83) contained in apoptotic infected fibroblasts is cross-presented to CD8 (+) T cells by dendritic cells显示文摘 | ARRODE G BOCCACCIO C LULE J | 2000 | J Virol2000，74，10： | 1 |
| 9 | Mechanical behavior of an osteotomized mandible with distraction ortho- dontic devices 显示文摘 | Boccaccio A Lamberti L Pappaletere C | 2006 | J Biomech2006，39，15： | 1 |
| 10 | The MET oncogene drives a genetic programme linking cancer to haemostasis显示文摘 | Boccaccio C Sabatino G Medico E | 2005 | Nature2005，434，7031： | 1 |
| 11 | Mechani-cal behaviour of an osteotomized mandible with distractionorthodontic devices显示文摘 | Boccaccio A Lamberti L Pappalettere C | 2006 | J Biomech2006，39，15： | 1 |
| 12 | Wesen CA Inguinal hernia recurrence followin preperitoneal Kugel patchrepair显示文摘 | Schroder DM Lloyd LR Boccaccio JE | 2004 | Am Surg2004，70，2： | 1 |
| 13 | A differentiation switch for genetically modified hepatocytes显示文摘 | Boccaccio C Ando M ComoglioPM | 2002 | FASEB J2002，16，1： | 1 |
| 14 | The influence ofexpansion rates on mandibular distraction osteogenesis:acomputational analysis显示文摘 | Boccaccio A Pappalettere C Kelly DJ | 2007 | Ann Biomed Eng2007，35，11： | 1 |
| 15 | Induction of epithe- lial tubules by growth factor HGF depends on the STAT pathway 显示文摘 | Boccaccio C Andb M Tamagnone L | 1998 | Nature1998，391，6664： | 1 |
| 16 | A mechano-regula- tion model of fracture repair in vertebral bodies显示文摘 | Boccaccio A Kelly D J Pappalettere C | 2011 | J Orthop Res2011，29，3： | 1 |
| 17 | Cancer and blood coagulation显示文摘 | Boccaccio C Medico E | 2006 | Cell Mol Life Sci2006，63，9： | 1 |
| 18 | Invasive growth: a MET-driven genetic programme for cancer and stem cells显示文摘 | Boccaccio C Comoglio P M | 2006 | Nat Rev Cancer2006，6，8： | 1 |
| 19 | Mechanical behav-ior of an osteotomized mandible with distraction orthodontic devices 显示文摘 | Boccaccio A Lamberti L Pappalettere C | 2006 | J Biomech2006，39，15： | 1 |
| 20 | Analysis of the performance of different orthodontic devices for mandibular symphyseal distraction osteogenesis显示文摘 | Boccaccio A Cozzani M Pappalettere C | 2011 | Eur J Orthod2011，33，2： | 1 |
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