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| 1 | No evidence of HPV DNA in esophageal squamous cell carcinoma in a population of Southern Brazil显示文摘AIM:To investigate the association between human papillomavirus(HPV)and esophageal squamous cell carcinoma(ESCC)in southern Brazil.METHODS:We studied 189 esophageal samples from125 patients from three different groups:(1)102 biopsies from 51 patients with ESCC,with one sample from the tumor and another from normal esophageal mucosa distant from the tumor;(2)50 esophageal biopsies from 37 patients with a previous diagnosis of head and neck squamous cell carcinoma(HNSCC);and(3)37 biopsies from esophageal mucosa with normal appearance from 37 dyspeptic patients,not exposed to smoking or alcohol consumption.Nested-polymerase chain reaction(PCR)with the MY09/11 and GP5/6 L1primers was used to detect HPV L1 in samples fixed in formalin and stored in paraffin blocks.All PCR reactions were performed with a positive control(cervicovaginal samples),with a negative control(Human Genomic DNA)and with a blank reaction containing all reagents except DNA.We took extreme care to prevent DNA contamination in sample collection,processing,and testing.RESULTS:The histological biopsies confirmed the diagnosis of ESCC in 52 samples(51 from ESCC group and 1 from the HNSCC group)and classified as well differentiated(12/52,23.1%),moderately differentiated(27/52,51.9%)or poorly differentiated(7/52,13.5%).One hundred twenty-eight esophageal biopsies were considered normal(51 from the ESCC group,42 from the HNSCC group and 35 from dyspeptic patients).Nine had esophagitis(7 from the HNSCC and 2 from dyspeptic patients).Of a total of 189 samples,only 6 samples had insufficient material for PCR analysis:1 from mucosa distant from the tumor in a patient with ESCC,3from patients with HNSCC and 2 from patients without cancer.In 183 samples(96.8%)GAPDH,G3PDH and/orβ-globin were amplified,thus indicating the adequacy of the DNA in those samples.HPV DNA was negative in all the 183 samples tested:52 with ESCC,9 with esophagitis and 122 with normal esophageal mucosa.CONCLUSION:There was no evidence of HPV infection in different ESCC from southern Brazil. | Luís Carlos Moreira Antunes Joo Carlos Prolla Antonio de Barros Lopes Marta Pires da Rocha Renato Borges Fagundes | 2013 | World Journal of Gastroenterology2013，19，39： | 4 |
| 2 | Perspectives on new biomarkers in gastric cancer: Diagnostic and prognostic applications显示文摘Gastric cancer is considered one of the most deadly tumors worldwide. Even with the decline in its incidence, the mortality rate of this disease has remained high, mainly due to its late diagnosis and to the lack of precise prognostic markers. The main purpose of this review is to present genetic, epigenetic and proteomic molecular markers that may be used in a diagnostic and prognostic manner and to discuss the pros and cons of each type of marker for improving clinical practice. In this sense, we observed that the use of genetic markers, especially mutations and polymorphisms, should be carefully considered, as they are strongly affected by ethnicity. Proteomic-based markers show promise, but the higher costs of the associated techniques con-tinue to make this approach expensive for routine use. Alternatively, epigenetic markers appear to be very promising, as they can be detected in bodily fluids as well as tissues. However, such markers must be used carefully because epigenetic changes may occur due to environmental factors and aging. Despite the advances in technology and its access, to date, there are few defined biomarkers of prognostic and diagnostic use for gastric tumors. Therefore, the use of a panel of several approaches(genetic, epigenetic and proteomic) should be considered the best alternative for clinical practice. | Danilo do Rosário Pinheiro Wallax Augusto Silva Ferreira Mariceli Baia Leo Barros Mariana Diniz Araújo Symara Rodrigues-Antunes Bárbara do Nascimento Borges | 2014 | World Journal of Gastroenterology2014，20，33： | 4 |
| 3 | Gut-microbiome-brain axis:the crosstalk between the vagus nerve,alpha-synuclein and the brain in Parkinson's disease显示文摘This critical review of the literature shows that there is a close link between the microbiome,the gut,and the brain in Parkinson's disease.The vagus nerve,the main component of the parasympathetic nervous system,is involved in the regulation of immune response,digestion,heart rate,and control of mood.It can detect microbiota metabolites through its afferents,transferring this gut information to the central nervous system.Preclinical and clinical studies have shown the important role played by the gut microbiome and gut-related factors in disease development and progression,as well as treatment responses.These findings suggest that the gut microbiome may be a valuable target for new therapeutic strategies for Parkinson's disease.More studies are needed to better understand the underlying biology and how this axis can be modulated for the patient's benefit. | Júlio César Claudino dos Santos Leandro Freitas Oliveira Felipe Micelli Noleto Camilla Teixeira Pinheiro Gusmão Gerly Anne de Castro Brito Glauce Socorro de Barros Viana | 2023 | Neural Regeneration Research2023，18，12： | 2 |
| 4 | Efficacy of CFRP- based techniques for the flexural and shear strengthening of concrete beams 显示文摘 | Barros J A O Dias S J E Lima J 1 T | 2007 | Cement and Concrete Composites2007，29，3： | 1 |
| 5 | Genetic varia bility of hepatitis B and C viruses in Brazilian patients with and without hepatocellular carcinoma显示文摘 | Araujo OC Barros JJ do O KM | 2014 | J Med Virol2014，86，2： | 1 |
| 6 | An investigation of the differential performance of clones of the aphid Sitobion avenae on two host species显示文摘 | BARRO PJ de SHERRATT T N DAVID O | 1995 | Oecologia1995，104，3： | 1 |
| 7 | Convergence Across States and Regions 显示文摘 | Barro R J Sala-i-Martin X Blanchard O J | 1991 | Brookings papers on Economic Activity1991，1991，1： | 1 |
| 8 | 查看详情显示文摘 | Barros V P Faria A L M acLeod T C O | | 0，，： | 1 |
| 9 | Isolation, identification, and activity in vitro of killer yeasts against Colletotrichum gloeosporioides isolated from tropical fruits显示文摘 | Jaqueline Rabelo de Lima Luciana Rocha Barros Gon?alves Luciana Rocha Brand?o Carlos Augusto Rosa Francisco Marto Pinto Viana | 2013 | J Basic Microbiol2013，，7： | 1 |
| 10 | Genetic variation within a parthenogenetic lineage显示文摘 | LUSHAI G BARRO PJ DAVID O | 1998 | Insect Molecular Biology1998，7，4： | 1 |
| 11 | Effects of the vitamin E in catalase activities in hippocampus after status epi- lepticus induced by pilocarpine in wistar rats显示文摘 | Barros D O Xavier S M L Barbosa C O etal | 2007 | Neuroscencei Letters2007，416，3： | 1 |
| 12 | Ovarian follicular dynamic in Nelore breed (Bos indicus) cattle显示文摘 | FIGUEIREDO R A BARROS C M PINHEIRO O L | | 0，，08： | 1 |
| 13 | Cat-transmitted sporotrichosis epidemic in Rio de Janeiro, Brazil: description of a series of cases 显示文摘 | Barros MB Schubach Ade O do Valle AC | 2004 | Clin Infect Dis2004，38，4： | 1 |
| 14 | An integrated approach for modelling the tensile behaviour of steel fibre reinforced self-compacting concrete 显示文摘 | Cunha V M C F Barros J A O Sena-Cruz J M | 2011 | Cement andConcreteResearch2011，41，1： | 1 |
| 15 | Clinical, etiological and progression factors of hearing in sudden deafness显示文摘 | Penido Nde O Ramos HV Barros FA | 2005 | Braz J Otorhinolaryngol2005，71，5： | 1 |
| 16 | Clinical, etiological and progression factors of hearing in sudden deafness 显示文摘 | Penido Nde O Ramos HV Barros FA | 2005 | Braz J Otorhinolaryngol2005，71，5： | 1 |
| 17 | A design model for fibre reinforced concrete beams pre-stressed withsteel and FRP bars 显示文摘 | BARROS J A O TAHERI M SALEHIAN H | 2012 | Composite structures2012，94，8： | 1 |
| 18 | Clinical,etiological and progression factors of hearingin sudden deafness显示文摘 | Penido Nde O Ramos HV Barros FA | 2005 | Rev Bras Otorrinolaringol2005，71，5： | 1 |
| 19 | Clinical, etiological and progression factors of hearing in sudden deafness 显示文摘 | Penido Nde O Ramos HV Barros FA | 2005 | Rev Bras Otorrinolaringol2005，71，5： | 1 |
| 20 | Microcalorimetric determination of the cell specific heat rate in soils: relationship with the soil microbial population and biophysic significance 显示文摘 | Barros N Feijóo S Fernández S | 2003 | Thermochim Acta2003，406，： | 1 |
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