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| 1 | 荧光多重实时PCR检测单纯疱疹病毒显示文摘目的建立单纯疱疹病毒的荧光多重实时PCR检测及分型技术,为生殖器疱疹的诊断及病毒分型提供快捷、敏感、特异的方法.方法以细胞培养法为对照,用Smart Cycler System作为实验平台,以DNA探针荧光标记技术建立荧光多重实时PCR,用于检测生殖器疱疹患者临床标本中的1型和2型单纯疱疹病毒(HSV1、HSV2)DNA.结果荧光多重实时PCR检测HSV1和HSV2 DNA的敏感性和特异性均为100%,可检测出低至1~5拷贝的HSV DNA,30 min左右即可完成整个检测过程,而细胞培养的敏感性和特异性分别为70.10%和100%.结论基于Smart Cycler System的荧光多重实时PCR是一种易于操作、扩增效率高、封闭式的核酸扩增技术,可用一次PCR反应就能快速、敏感、准确地对临床标本中的单纯疱疹病毒进行检测和分型. | 赖维 Cheng-Yen Chen 苏向阳 黄怀球 张玉清 Ron C Ballard Stephen A Morse | 2004 | 中华皮肤科杂志2004，37，5： | 6 |
| 2 | Prospective follow-up study between 3 and 15 months after stroke:improvements and decline in cognitive function among dementia-free stroke survivors > 75 years of age显示文摘 | Ballard C Rowan E Stephens S | 2003 | Stroke2003，34，10： | 1 |
| 3 | A comparative study of psychiatric symptoms in dementia with Lewy bodies and Parkinson's disease with and without dementia显示文摘 | Ballard C Larsen JP | 2001 | Int J Geriatr Psychiatry2001，16，： | 1 |
| 4 | Stages of embryonic development of the zebrafish显示文摘 | KIMMEL C B BALLARD W W KIMMEL S R | 1995 | Dev Dyn1995，203，： | 1 |
| 5 | Controlling a computer via facial aspect 显示文摘 | Ballard P Stockman G C | 1996 | IEEE Transactiones on Systems Man and Cybenetics1996，25，4： | 1 |
| 6 | Opti- mised anaesthesia to reduce post operative cognitive decline (POCD)in older patients undergoing elective surgery,a randomisedcontrolled trial显示文摘 | Ballard C Jones E Gauge N | 2012 | PLoS 0ne2012，7，37： | 1 |
| 7 | Y chromosome STR haplotypes in three UK populations显示文摘 | Ballard DJ Phillips C Thacker CR | 2005 | Forensic Sci Int2005，152，23： | 1 |
| 8 | Compari- son of testoster-one metabolism in benign p rostatic hy- perp lasia and human prostate cancer cell lines in vitro 显示文摘 | Smith C M Ballard S A Wyllie M G | 1994 | J Steroid Biochem Mol Biol1994，50，34： | 1 |
| 9 | Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos显示文摘 | Giraldo A M Hylan D A Ballard C B | 2008 | Biol Reprod2008，78，5： | 1 |
| 10 | Comparison of corn silage hybrids for yield,nutrient composition,in vitro digestibility,and milk yield by dairy cows显示文摘 | Thomas E D Mandebvu P Ballard C S | 2001 | Journal of Animal Science2001，84，： | 1 |
| 11 | Mechanisms underlying depressed Na^+/Ca^2+ exchanger activity in the diabetic heart 显示文摘 | Schaffer S W Ballard C C Boerth S | 1997 | Cardiovasc Res1997，34，1： | 1 |
| 12 | Annual report to the nation on the status of cancer, 1975-2008, featuring cancers associated with excess weight and lack of sufficient physical activity显示文摘 | Eheman C Henley SJ Ballard BR | 2012 | Cancer2012，118，9： | 1 |
| 13 | Magnetite pathogenesis and digenetic palate premature across the northern Appalachian basin显示文摘 | Jackson M Mccabe C Ballard M | 1988 | Geology1988，16，： | 1 |
| 14 | Optimised anaesthesia to reducepost operative cognitive decline(POCD)in older patients undergoingelective surgery,a randomised controlled trial显示文摘 | Ballard C Jones L Gauge N | 2012 | PLoS One2012，7，37： | 1 |
| 15 | Cholinesterase inhibitom reduce cor-tical Abeta in dementia with Lewy bodies显示文摘 | Ballard CG Chalmers KA Todd C | 2007 | Neurology2007，68，20： | 1 |
| 16 | A positive association be- tween 5-HT re-uptake binding sites and depression in dementia with Lewy bodies显示文摘 | Ballard C Johnson M Piggott M | 2002 | Affect Disord2002，69，13： | 1 |
| 17 | The incomplete natural history of mitochondria显示文摘 | Ballard J W O Whitlock M C | 2004 | Molecular Ecology2004，13，： | 1 |
| 18 | A comparison of medial and lateral temporal lobe atrophy in dementia with Lewy bodies and Alzheimer's disease: magnetic resonance imaging volumetic study显示文摘 | McKeith IG Ballard C | 2001 | Dement Geriatr Cogn Disorder2001，12，： | 1 |
| 19 | Stages of embryonic development of the Zebra fish显示文摘 | Kimmel C B Ballard W W Kimmel S R | 1995 | Developmental Dynamics1995，203，： | 1 |
| 20 | Depression in Parkinson disease-epidemiology,mechanisms and management显示文摘 | Aarsland D P hlhagen S Ballard C G | 2012 | Nature Reviews Neurology2012，8，1： | 1 |
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