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| 1 | Rapid and Cost-Effective Gene Targeting in Rat Embryonic Stem Cells by TALENs显示文摘老鼠是在生物医学的研究和药开发的许多区域的比较喜欢的动物模型。在老鼠的基因操作由于指向工具的有效基因的缺乏在老鼠落后于那。以前,我们在老鼠经由常规相应再结合产生了一只猛烈老鼠胚胎的茎(ES ) 细胞。这里,我们证明在老鼠 ES 房间指向的那有效基因能通过抄写快速被完成像使活跃之物的受动器核酸酶(TALEN ) 调停了 DNA 双海滨裂缝。用克隆技术的金门,我们构造了为对 5 天的兴趣的基因指向向量的一双 TALEN。在基因 transfection 以后,指向的老鼠 ES 房间殖民地被没有药选择的需要的 PCR 孤立,屏蔽,并且证实。我们的结果建议调停 TALEN 的基因指向是与高效率和短重组时间建立遗传上修改的老鼠 ES 房间线的一个优异工具。 | Charles Ashton | 2012 | Journal of Genetics and Genomics2012，39，6： | 21 |
| 2 | Expansion and differentiation of human hepatocyte-derived liver progenitor-like cells and their use for the study of hepatotropic pathogens显示文摘The study of pathophysiological mecha nisms in huma n liver disease has bee n constrained by the in ability to expa nd primary hepatocytes in vitro while maintaining proliferative capacity and metabolic function. We and others have previously shown that mouse mature hepatocytes can be con verted to liver progenitor-like cells in vitro with defined chemical factors. Here we describe a protocol achieving efficient conversion of human primary hepatocytes into liver progenitor-like cells (HepLPCs) through delivery of developmentally relevant cues, including NAD +-dependent deacetylase SIRT1 signaling. These HepLPCs could be expanded sign ifica ntly duri ng in vitro passage. The expa nded cells can readily be con verted back into metabolically functional hepatocytes in vitro and upon tran splantati on in vivo. Under three-dime nsional culture conditions, differentiated cells gen erated from HepLPCs regai ned the ability to support infect io n or reactivatio n of hepatitis B virus (HBV). Our work dem on strates the utility of the con versi on betwee n hepatocyte and liver proge nitor-like cells for studying HBV biology and an tiviral therapies. These fin dings will facilitate the study of liver diseases and regenerative medicine. | Gong-Bo Fu Wei-Jian Huang Min Zeng Xu Zhou Hong-Ping Wu Chang-Cheng Liu Han Wu Jun Weng Hong-Dan Zhang Yong-Chao Cai Charles Ashton Min Ding Dan Tang Bao-Hua Zhang Yi Gao Wei-Feng Yu Bo Zhai Zhi-Ying He Hong-Yang Wang He-Xin Yan | 2019 | Cell Research2019，29，1： | 18 |
| 3 | Reversible transition between hepatocytes and liver progenitors for in vitro hepatocyte expansion显示文摘 | Wu, Han Zhou, Xu Fu, Gong-Bo He, Zhi-Ying Wu, Hong-Ping You, Pu Ashton, Charles Wang, Xin Wang, Hong-Yang Yan, He-Xin | 2017 | Cell Research2017，27，5： | 12 |
| 4 | Characterization of Gibberellin Receptor Mutants of Barley (Hordeum vulgare L.)显示文摘Gid1 的顺序(为赤霉素(GA ) 的基因从米饭的受体) 被用来从大麦识别通常认为的 orthologue。这在 E 被表示。coli,并且生产了能与结构的特性和 saturability 在 vitro 绑 GA 的蛋白质。它在 GA 回答的潜在的角色与减少的 GA 敏感(gse1 异种) 用大麦异种被调查。十六不同 gse1 异种各在这个序列带了唯一的核苷酸替换。在里面几乎一个案例,这些变化导致了单个氨基酸替换,并且,为留下的异种,在 mRNA 的 5 untranslated 区域的替换被建议防碍翻译开始。在分离有完美的连接在新变异的等位基因和 gse1 显型之间的人口,导致在大麦的通常认为的 GID1 GA 受体顺序对应于 Gse1 地点的结论。在异种之一的内长的 GA 内容的决心揭示了 C20 GA 先锋的 bioactive GA1,和赤字的提高的累积。所有在通常正在浸透的集中 gse1 异种把敏感归结为外长的 GA3,并且到 AC94377 (GA 类似物) ,,但是,在高得多的集中,经常有可观的回答。在大麦和米饭异种之间的比较揭示这之间的有趣的差别在 GA 的二谷物种神经质的生理学。 | Peter M. Chandler Carol A. Harding Anthony R. Ashton Mark D. Mulcair Nicholas E. Dixonb Lewis N. Manderb | 2008 | Molecular Plant2008，1，2： | 10 |
| 5 | The tropical-subtropical evergreen forest transition in East Asia:An exploration显示文摘The transition from tropical to subtropical(warm temperate)evergreen forests is more clearly apparent in East Asia,fromNepal to the western Pacific coast,than elsewhere in the tropics.We review the nature of this transition and hypothesize the physical,ultimately climatic,factors thatmay maintain it,with a special focus on how the increasing instability and warming of climates will affect these forests.A primary climatic mediator of the transition is proposed,thereby offering a testable hypothesis for the climateeforest transition relationship.What is known of this transition is summarized in context of the primary climatic mediators of elevational zonation of forest formations in equatorial Asia to the tree line,in the Himalaya at the India-Indo-Burma northern tropical margin,and as both elevational and latitudinal zonation in southern China.Consequent secondary edaphic and other physical changes are described for the Himalaya,and hypothesized for southern China.The forest ecotones are seen to be primarily defined by tree floristic change,on which account changes in structure and physiognomy are determined.The montane tropical-subtropical transition in the Himalaya is narrowand observed to correlate with an as yet ill-defined frost line.A distinct tropical-subtropical transition forest is recognized in the southwest Chinamountains.There is a total change in canopy species at the Himalayan ecotone,but subcanopy tropical species persist along an elevational decline of c.400 m.The latitudinal transition in South China is analogous,but here the tropical subcanopy component extends north over ten degrees latitude,albeit in decline.The tropical-subtropical transition is uniquely clear in East Asia because here alone a tropical wet summer-dry winter monsoon extends to 35north latitude,encompassing the subtropical evergreen forest,whereas subtropical evergreen forests elsewhere exist under drier temperate summer climate regimes. | Peter Ashton Hua Zhu | 2020 | Plant Diversity2020，42，4： | 8 |
| 6 | 中国热带-亚热带常绿阔叶林群落交错区显示文摘在中国南部热带北缘,热带森林向亚热带常绿阔叶林转变过渡,它们之间存在群落交错区.本文主要通过对中国热带北缘地区的4个永久样地植被的群落结构、生态外貌和植物区系组成分析,论证了热带-亚热带常绿阔叶林群落交错区的存在和其识别特征.由于中国南部西高东低的地势,西南部群落交错区主要体现在沿海拔的转变过渡,东南部则是沿纬度的转变过渡.西南部热带北缘存在两个明显的群落交错区.在海拔800~1200 m,热带低地季节性雨林与热带低山常绿阔叶林交错过渡,森林群落的外貌、结构和林冠层树种明显改变:由具有3个乔木亚层和散生巨树、典型混交热带树种组成林冠层的热带低地季节性雨林转变为具有2个乔木亚层、缺乏散生巨树、林冠趋于平整、主要由热带山地分布的壳斗科和茶科植物为林冠层优势树种的热带低山常绿阔叶林.在海拔1800~2100(2200)m,热带低山常绿阔叶林向亚热带常绿阔叶林交错过渡,林冠层树种发生了替换,群落的植物区系性质也发生了改变,由热带性质的植物区系变成亚热带-暖温带性质的植物区系.在中国东南部低地,约22°30′N及两侧附近,存在一个明显的常绿森林群落交错区:热带北缘低地季节性雨林向(南)亚热带低地常绿阔叶林交错过渡,群落的外貌、结构和林冠层树种明显改变.我们认为,热带-亚热带常绿阔叶林群落交错区与霜冻情况有关,霜冻对热带低地季节性雨林的林冠树种是致命的.我们建议,西南部的热带低山常绿阔叶林与亚热带常绿阔叶林的群落交错区和东南部的低地热带季节性雨林与亚热带常绿阔叶林的群落交错区为热带森林分布的北界.超越热带北界,林冠树种在西南部替换为亚热带-温带分布的中国-喜马拉雅和中国特有种,在东南部替换为主要是亚热带分布的中国特有种.中国东南部的亚热带气候地区存在热带植物区系成分,本文也对其原因进行了探讨,并提出了尚需进一步研究的问题. | 朱华 Peter Ashton | 2021 | 科学通报2021，66，28： | 7 |
| 7 | AJCN:过敏症来袭怎么办?新型益生菌组合疗法显神效显示文摘当进入过敏季节我们可能并不太会拿出手帕或者打喷嚏来预防过敏。日前,一项刊登在国际杂志The American Journal of Clinical Nutrition上的研究报告中。来自佛罗里达大学的研究人员通过研究发现。一种益生菌组合或许能够帮助减轻花粉症的症状。 | Jennifer C Dennis-Wall Tyler Culpepper Carmelo Nieves Jr. Cassie C Rowe Alyssa M Burns Carley T Rusch Ashton Federico Maria Ukhanova Sheldon Waugh Volker Mai Mary C Christman Bobbi Langkamp-Henken | 2017 | 现代生物医学进展2017，17，18： | 3 |
| 8 | Biological conversion of low-grade coal discard to a humic substance-enriched soil-like material显示文摘 | Lerato Mary Sekhohola Ashton Keith Cowan | 2017 | International Journal of Coal Science & Technology2017，4，2： | 3 |
| 9 | Novel Technique for All-Optical Clock Extraction Using Fiber Bragg Gratings显示文摘We demonstrate a simple passive technique, using phase shifted fibre Bragg gratings, for extraction of optical clock signals from RZ optical data signals. The generation of an optical clock signal at 12.5 GHz from a 12.5 Gbs RZ optical data stream is experimentally demonstrated with simulation of optical clock extraction at 10 GHz from 40 Gbs OTDM data streams. | M. Attygalle B. Ashton A. Nirmalathas L. Poladian W. Padden | 2003 | 光学学报2003，23，S1： | 2 |
| 10 | A simple scoring system to estimate perioperative mortality following liver resection for primary liver malignancy-the Hepatectomy Risk Score(HeRS)显示文摘Background:Selection of the optimal treatment modality for primary liver cancers remains complex,balancing patient condition,liver function,and extent of disease.In individuals with preserved liver function,liver resection remains the primary approach for treatment with curative intent but may be associated with significant mortality.The purpose of this study was to establish a simple scoring system based on Model for End-stage Liver Disease(MELD)and extent of resection to guide risk assessment for liver resections.Methods:The 2005-2015 NSQIP database was queried for patients undergoing liver resection for primary liver malignancy.We first developed a model that incorporated the extent of resection(1 point for major hepatectomy)and a MELD-Na score category of low(MELD-Na=6,1 point),medium(MELD-Na=7-10,2 points)or high(MELD-Na>10,3 points)with a score range of 1-4,called the Hepatic Resection Risk Score(HeRS).We tested the predictive value of this model on the dataset using logistic regression.We next developed an optimal multivariable model using backwards sequential selection of variables under logistic regression.We performed K-fold cross validation on both models.Receiver operating characteristics were plotted and the optimal sensitivity and specificity for each model were calculated to obtain positive and negative predictive values.Results:A total of 4,510 patients were included.HeRS was associated with increased odds of 30-day mortality[HeRS=2:OR=3.23(1.16-8.99),P=0.025;HeRS=3:OR=6.54(2.39-17.90),P<0.001;HeRS=4:OR=13.69(4.90-38.22),P<0.001].The AUC for this model was 0.66.The AUC for the optimal multivariable model was higher at 0.76.Under K-fold cross validation,the positive predictive value(PPV)and negative predictive value(NPV)of these two models were similar at PPV=6.4%and NPV=97.7%for the HeRS only model and PPV=8.4%and NPV=98.1%for the optimal multivariable model.Conclusions:The HeRS offers a simple heuristic for estimating 30-day mortality after resection of primary liver malignancy.More complicated models offer better performance but at the expense of being more difficult to integrate into clinical practice. | Dimitrios Moris Brian I.Shaw Cecilia Ong Ashton Connor Mariya L.Samoylova Samuel J.Kesseli Nader Abraham Jared Gloria Robin Schmitz Zachary W.Fitch Bryan M.Clary Andrew S.Barbas | 2021 | Hepatobiliary Surgery and Nutrition2021，10，3： | 2 |
| 11 | Tropical deciduous forest in Yunnan,southwestern China:Implications for geological and climatic histories from a little-known forest formation显示文摘In the southern mountain ranges of Yunnan province,China,deep valleys of several large rivers create rain shadows with hot dry summers,and are locally designated tropical;towards the north,notably in the Lancang(Upper Mekong)valley,these regions may experience frost during winter.The woody forest canopy of these valleys is predominantly deciduous,with evergreen elements in the north,where the canopy is open and the forest savanna-like.However,we here present tall forest with a closed deciduous canopy and semi-evergreen subcanopy observed in hot dry valleys of these rivers and their tributaries in the tropical south.The structure and physiognomy of these forests resemble the tall(moist)deciduous forest formation widespread in South Asia and Indo-Burma.Furthermore,these forests are largely composed of tropical elements at both the generic(80%)and the species level(>70%),indicating that these forests are indeed tropical.We originally hypothesized that these isolated forests represent refugia of a pre-Holocene extension of tall(moist)deciduous forest formation of South Asia and Indo-Burma.The sample plot we established to test this hypothesis confirmed that these forests share the structure and physiognomy of the tall(moist)deciduous forest formation;however,the plots also showed that these forests lack the characteristic and dominant species of the formation's Indo-Burmese range.The tree flora,in particular,indicates that both deciduous and evergreen elements are instead mostly derived from the adjacent tropical semi-evergreen forests of tropical southern China;yet they also include an important endemic element,which implies that these forests have survived as refuges possibly since the Pliocene.The exceptional representation of evergreen elements in these forests indicates that they have rarely been subject to hot fires or domestic cattle browsing,adding to the unique nature of the forests and further justifying their strict conservation. | Hua Zhu Peter Ashton Bojian Gu Shisun Zhou Yunhong Tan | 2021 | Plant Diversity2021，43，6： | 2 |
| 12 | Fluocinolone Acetonide Intravitreal Implant for Diabetic Macular Edema: A 3-Year Multicenter, Randomized, Controlled Clinical Trial显示文摘 | P. Andrew Pearson Timothy L. Comstock Michael Ip David Callanan Lawrence S. Morse Paul Ashton Brian Levy Eric S. Mann Dean Eliott | 2011 | Ophthalmology2011，，8： | 2 |
| 13 | Bacterial degradation of coal discard and geologically weathered coal显示文摘The biodegradation of coal discard is being intensively studied in South Africa in an effort to develop passive methods for the successful revegetation and rehabilitation of waste dumps, to mitigate pollution, and facilitate mine closure. Bacteria were isolated from slurries of coal tailings and diesel-contaminated soil, screened for coal biodegradation competence, characterized, and the colonization and degradation of coal discard and geologically weathered coal investigated using individual isolates and consortia. Ten novel coal-degrading bacterial strains were isolated and characterized, the gene sequences deposited with GenBank, and the (wild-type) strains deposited at Microbial Culture Collection, India. The results from the present work show that bituminous coal discard and geologically weathered coal is used by these isolates as carbon and energy source. Isolated strains and consortia colonized and degraded both coal substrates. Growth rate of the isolates is faster and stationery phase achieved sooner in minimal medium containing geologically weathered coal. This observation suggests that the oxygen-rich weathered coal is a more friable substrate and thus readily colonised and biodegraded. A reduction in mass of substrate is demonstrated for both individual isolates and consortia. The changes in pH and associated media colouration occurred concomitant with formation of humic acid-like (HS) and fulvic acid-like substances (FS) which is confirmed following analysis of these products by FT-IR spectroscopy. It is concluded that preferential metabolism of alkanes from the coal substrates provided the carbon and energy for bacterial growth and transformation of the substrates to HS and FS. | Jacob Taiwo Olawale Oghenekume Gerald Edeki Ashton Keith Cowan | 2020 | International Journal of Coal Science & Technology2020，7，2： | 2 |
| 14 | The functional anatomy of the female pelvic floor and stress continence control system 显示文摘 | Ashton - Miller Howard D DaLancey OL | 2001 | Scand J Urol Nephrol2001，207，： | 1 |
| 15 | Ultrasonic attenuation in voided fibre - reinforced plastics显示文摘 | Hale J M Ashton J N | 1988 | NDT International1988，21，5： | 1 |
| 16 | Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing 显示文摘 | Chamberlain G Fox J Ashton B | 2007 | Stem Cdls2007，25，11： | 1 |
| 17 | An intravitreal device providing sustained release of cyclosporine and dexamethasone显示文摘 | Enyedi LB Pearson PA Ashton P Jaffe GJ | 1996 | Curr Eye Res1996，15，： | 1 |
| 18 | Association between blood pressure: the treatment of hypertension,and cardiovascular risk factors in women 显示文摘 | Nanchahal K Ashton WD Wood DA | 2000 | J Hypertens2000，18，： | 1 |
| 19 | Self-assembly of rotaxanes bearing dendritic stopper显示文摘 | Amabilino D A Ashton P R Balzani V | 1996 | J Am Chem Soc1996，118，12： | 1 |
| 20 | A feasibility study of the use of random amplified polymorphic DNA in the population genetics and systematics of grasshoppers显示文摘 | Chapco W Ashton NW Martel RK Antonishyn N Crosby WL | 1992 | Genome1992，35，4： | 1 |
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