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| 1 | Cytotoxic genes from traditional Chinese medicine inhibit tumor growth both in vitro and in vivo显示文摘OBJECTIVE: Little effort has been made to study the protein-encoding genes isolated from traditional Chinese medicine(TCM) drugs, and the delivery of these genes into malignant cells through recombinant adeno-associated virus(r AAV) vectors has not been attempted. METHODS: We synthesized the c DNAs of five known cytotoxic proteins isolated from TCM drugs and the FLAG epitope-tagged c DNAs were subcloned into a r AAV plasmid vector. The protein expression was confi rmed by Western blot assay. Various cancer cell lines were transfected with the above plasmids and cell growth was monitored both in vitro and in vivo. The best cytotoxic gene was further packaged into r AAV vectors, under the control of a liver cancer-specifi c promoter. The liver tumor growth was then monitored following intratumor administration of the r AAV vectors.RESULTS: The expression plasmids, encoding individual potential cytotoxic genes tagged with FLAG epitope, were successfully generated and sequenced. Among these genes, trichosanthin(TCS) gene yielded the most promising results for the inhibition of cancer cell growth in vitro. The over-expressed TCS functioned as a type I ribosome-inactivating protein, followed by inducing apoptosis that is associated with the Bcl-PARP signaling pathway. Furthermore, intratumor injection of r AAV vectors containing the TCS gene signifi cantly inhibited the growth of human hepatocellular carcinoma tumors in a murine xenograft model.CONCLUSION: Our studies suggest that the use of TCM cytotoxic genes is a useful therapeutic strategy for treating human cancers in general, and liver tumors in particular. | Yuan-hui Zhang Yuan Wang Ali Hussein Yusufali Frederick Ashby Daniel Zhang Zi-fei Yin George V.Aslanidi Arun Srivastava Chang-quan Ling Chen Ling | 2014 | Journal of Integrative Medicine2014，12，6： | 14 |
| 2 | 恶性胶质瘤的治疗:综合方案的进展显示文摘第一部分 中枢神经系统肿瘤(central nerous system tumors)
一、流行病学特征(epidemiological features)
中枢神经系统原发肿瘤,从高度恶性胶质瘤和原始神经外胚层肿瘤到良性脑膜瘤、神经瘤及垂体腺瘤,可表现出不同的生物学行为。根据来自美国脑肿瘤注册中心(Central Brain Tumor Registry of the United States,CBTRUS)的最新报告,每年新诊断的原发脑恶性肿瘤的人数为7.30/10万人。美国脑肿瘤注册中心2005年报告新诊断的中枢神经系统原发肿瘤患者为21690例,死亡12760例。胶质瘤占神经系统原发肿瘤的42%,占恶性肿瘤的77%。胶质瘤起源于不同的组织类型,包括少突胶质细胞瘤、星形细胞瘤、混合型少突星形细胞瘤,表现为不同的恶性程度,而且均具有向高度恶性转化的趋势,其中多形性胶质母细胞瘤(glioblasto mamultiforme,GBM)为成人中最常见的并具有浸润性的原发脑肿瘤。 | Lynn S Ashby(著) Timothy C Ryken(著) 刘暌(译) 只达石(校) | 2006 | 中国现代神经疾病杂志2006，6，6： | 8 |
| 3 | 药物性肝损伤恢复期长与发病时高胆红素、高碱性磷酸酶及药物代谢相关显示文摘【据Journal of Hepatology 2021年4月报道】药物性肝损伤恢复期长与发病时高胆红素、高碱性磷酸酶及药物代谢相关(作者Ashby K等)尽管大多数药物性肝损伤(DILI)在停止使用致病药物后预后良好,但患者的恢复期各不相同,6%~12%的患者会发生慢性化。基于此,该研究调查了决定DILI恢复时间的潜在危险因素如临床因素和药物特性,并开发出预测DILI恢复时间的模型。 | 张飞宇 高沿航 ASHBY K ZHUANG W GONZALEZ-JIMENEZ A | 2021 | 临床肝胆病杂志2021，37，5： | 7 |
| 4 | Cancer chemoprevention through dietary avonoids: what's limiting?显示文摘Flavonoids are polyphenols that are found in numerous edible plant species. Data obtained from preclinical and clinical studies suggest that specific flavonoids are chemo-preventive and cytotoxic against various cancers via a multitude of mechanisms. However, the clinical use of flavonoids is limited due to challenges associated with their e ective use, including(1) the isolation and purification of flavonoids from their natural resources;(2) demonstration of the e ects of flavonoids in reducing the risk of certain cancer, in tandem with the cost and time needed for epidemiological studies, and(3) numerous pharmacokinetic challenges(e.g., bioavailability, drug–drug interactions, and metabolic instability). Currently, numerous approaches are being used to surmount some of these challenges, thereby increasing the likelihood of flavonoids being used as chemo-preventive drugs in the clinic. In this review, we summarize the most important challenges and e orts that are being made to surmount these challenges. | Haneen Amawi Charles R. Ashby Jr Amit K. Tiwari | 2017 | Chinese Journal of Cancer2017，36，10： | 4 |
| 5 | ATP-binding cassette subfamily B member 1 (ABCB1) and subfamily C member 10(ABCC1O) are not primary resistance factors for cabazitaxel显示文摘Introduction:ATP-binding cassette subfamily B member 1(ABCB1) and subfamily C member 10(ABCCIO) proteins are efflux transporters that couple the energy derived from ATP hydrolysis to the translocation of toxic substances and chemotherapeutic drugs out of cells.Cabazitaxel is a novel taxane that differs from paclitaxel by its lower affinity for ATP-binding cassette(ABC) transporters.Methods:We determined the effects of cabazitaxel,a novel tubulin-binding taxane,and paclitaxel on paclitaxelresistant,ABCB1-overexpressing KB-C2 and LLC-MDR1-WT cells and paclitaxel-resistant,ABCC10-overexpressing HEK293/ABCC10 cells by calculating the degree of drug resistance and measuring ATPase activity of the ABCB1 transporter.Results:Decreased resistance to cabazitaxel compared with paclitaxel was observed in KB-C2,LLC-MDR1-WT,and HEK293/ABCC10 cells.Moreover,cabazitaxel had low efficacy,whereas paclitaxel had high efficacy in stimulating the ATPase activity of ABCB1,indicating a direct interaction of both drugs with the transporter.Conclusion:ABCB1 and ABCC10 are not primary resistance factors for cabazitaxel compared with paclitaxel,suggesting that cabazitaxel may have a low affinity for these efflux transporters. | Rishil J Kathawala Yi-Jun Wang Suneet Shukla Yun-Kai Zhang Saeed Alqahtani Amal Kaddoumi Suresh V Ambudkar Charles R Ashby Jr Zhe-Sheng Chen | 2015 | Chinese Journal of Cancer2015，34，3： | 4 |
| 6 | Carbon dioxide insufflation during colonoscopy in deeply sedated patients显示文摘AIM:To compare the impact of carbon dioxide(CO2) and air insufflation on patient tolerance/safety in deeply sedated patients undergoing colonoscopy.METHODS:Patients referred for colonoscopy were randomized to receive either CO2 or air insufflation during the procedure.Both the colonoscopist and patient were blinded to the type of gas used.During the procedure,insertion and withdrawal times,caecal intubationrates,total sedation given and capnography readings were recorded.The level of sedation and magnitude of patient discomfort during the procedure was assessed by a nurse using a visual analogue scale(VAS)(0-3).Patients then graded their level of discomfort and abdominal bloating using a similar VAS.Complications during and after the procedure were recorded.RESULTS:A total of 142 patients were randomized with 72 in the air arm and 70 in the CO2 arm.Mean age between the two study groups were similar.Insertion time to the caecum was quicker in the CO2 group at 7.3 min vs 9.9 min with air(P = 0.0083).The average withdrawal times were not significantly different between the two groups.Caecal intubation rates were 94.4% and 100% in the air and CO2 groups respectively(P = 0.012).The level of discomfort assessed by the nurse was 0.69(air) and 0.39(CO2)(P = 0.0155) and by the patient 0.82(air) and 0.46(CO2)(P = 0.0228).The level of abdominal bloating was 0.97(air) and 0.36(CO2)(P = 0.001).Capnography readings trended to be higher in the CO2 group at the commencement,caecal intubation,and conclusion of the procedure,even though this was not significantly different when compared to readings obtained during air insufflation.There were no complications in both arms.CONCLUSION:CO2 insufflation during colonoscopy is more efficacious than air,allowing quicker and better cecal intubation rates.Abdominal discomfort and bloating were significantly less with CO2 insufflation. | Rajvinder Singh Eu Nice Neo Nazree Nordeen Ganesananthan Shanmuganathan Angelie Ashby Sharon Drummond Garry Nind Elizabeth Murphy Andrew Luck Graeme Tucker William Tam | 2012 | World Journal of Gastroenterology2012，18，25： | 3 |
| 7 | Ipsilateral fast corticospinal pathways do not account for recovery in stroke显示文摘 | Palmer E Ashby P Hajek V | 1992 | Ann Neurol1992，32，： | 2 |
| 8 | Low-grade glioma: supratentorial astrocytoma, oligodendroglioma, and oligoastrocytoma in adults显示文摘 | Ashby LS Shapiro WR | 2004 | Curr Neurol Neurosci Rep2004，4，3： | 1 |
| 9 | Targeting tumor cell motility to prevent Metastasis 显示文摘 | Palmer riD Ashby WJ Lewis JD | 2011 | Advanced Drug Delivery Reviews2011，63，： | 1 |
| 10 | Antimicrobial resistance in Gram-positive pathogens isolated in the UK between October 1996 and January 1997显示文摘 | Andrews J Ashby J Jevons G | 1999 | J Antimicrob Chemother1999，43，： | 1 |
| 11 | Sophorulipid bio- synthesis from a biodiesel co-product stream显示文摘 | Ashby R D Nunez A Solaiman D K Y | 2005 | J Am Oil Chem Soc2005，82，： | 1 |
| 12 | BCR-ABL tyrosine kinase inhibitors in the treatment of Philadelphia chromosome positive chronic myeloid leukemia: A review显示文摘 | Xin An Amit K. Tiwari Yibo Sun Pei-Rong Ding Charles R. Ashby Zhe-Sheng Chen | 2010 | Leukemia Research2010，，10： | 1 |
| 13 | The effect of bright light on lens compensation in chicks显示文摘 | Ashby RS Schaeffel F | 2010 | Invest Ophthalmol Vis Sci2010，51，10： | 1 |
| 14 | The effect on sperm production in adult Sprague-Dawley rats exposed by gavage to bisphenol A between postnatal days 91-97显示文摘 | Ashby J Tinwell H Lefevre PA | 2003 | Toxicol Sci2003，74，： | 1 |
| 15 | The failure of brittle solids containing small cracks under compressive stress states 显示文摘 | ASHBY M F HALLAM S D | 1986 | Acta Metall1986，34，： | 1 |
| 16 | The mechanical properties of cellular solids 显示文摘 | Ashby M F | 1983 | Metall Trans1983，14，： | 1 |
| 17 | The medical management of ectopic pregnancy:a meta-analysis comparing 'single dose' and 'multidose' regimens显示文摘 | Barnhart KT Gosman G Ashby R | 2003 | Obstet Gynecol2003，101，4： | 1 |
| 18 | The medical management of ectopic pregnancy: a meta-analysis comparing 'single dose' and 'multidose' regimens显示文摘 | Barnhart KT Gosman G Ashby R | 2003 | Obstet Gynecol2003，101，4： | 1 |
| 19 | The effect of ambient illuminance on the development of deprivation myopia in chicks 显示文摘 | Ashby R Ohlendorf A Schaeffel F | 2009 | Invest Ophthalmol Vis Sci2009，50，： | 1 |
| 20 | The effect of ambient illuminance on the development of deprivation myopia in chicks显示文摘 | Ashby R Ohlendorf A Schaeffel F | | 0，，： | 1 |
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