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| 1 | 龙须菜(Gracilaria lemaneiformis)选育品系及其野生型的ISSR指纹分析显示文摘采用ISSR标记对龙须菜一个野生型群体和选育品系两个不同年代的栽培群体,进行了亲缘关系分析,构建了龙须菜选育品系的指纹图谱。通过实验从22个ISSR引物中筛选出16条引物,可以产生清晰稳定及可重复的带,共扩增出118个位点;野生型和选育品系两个群体的遗传相似率分别为0.7301、0.7189,选育品系的两养殖种群间的遗传相似率为0.9375;遗传距离聚类分析证明,龙须菜选育品系与其青岛野生型亲缘关系很近。7条引物产生的12条特异片段可用于龙须菜选育品系的种质鉴定,每条引物都能将龙须菜选育品系和其野生型分开;根据引物S848扩增的位点构建了指纹图谱,可用于区分龙须菜选育种群及野生种群。 | 李文红 姚建亭 王继成 王如才 段德麟 | 2005 | 海洋与湖沼2005，36，3： | 20 |
| 2 | Effects of salinity and nutrients on the growth and chlorophyll fluorescence of Caulerpa lentillifera显示文摘Caulerpa lentillifera is a green algae that distributes worldwide and is cultivated for food. We assessed vegetative propagation of C. lentillifera by measuring the specific growth rate(SGR) and chlorophyll fluorescence of the green algae cultured at different salinities and nutrient levels. The results indicated that C. lentillifera can survive in salinities ranging from 20 to 50,and can develop at salinities of 30 to 40. The maximum SGR for C. lentillifera occurred at a salinity of 35. Both chlorophyll content and the ratio of variable to maximum fluorescence( Fv / Fm) were also at a maximum at a salinity of 35. Photosynthesis was inhibited in salinities greater than 45 and less than 25. Both the maximum SGR and maximum chlorophyll content were found in algae treated with a concentration of 0.5 mmol/L of NO3-N and 0.1 mmol/L of PO 4-P. The photosynthetic capacity of photosystem II(PSII) was inhibited in cultures of C. lentillifera at high nutrient levels. This occurred when NO3-N concentrations were greater than 1.0 mmol/L and when PO4- P concentrations were at 0.4 mmol/L. As there is strong need for large-scale cultivation of C. lentillifera,these data contribute important information to ensure optimal results. | 郭辉 姚建亭 孙忠民 段德麟 | 2015 | Chinese Journal of Oceanology and Limnology2015，33，2： | 7 |
| 3 | Effect of temperature and irradiance on the growth and reproduction of Enteromorpha prolifera J.Ag.(Chlorophycophyta,Chlorophyceae)显示文摘开花的 Enteromorpha prolifera 的生长和复制上的温度和发光的效果沿着在夏天 2008 的 Qingdao 海岸近海,被学习。它被看那 E。在 25 ° C /40 μmol m −2 s −1 。在这个条件下面,有二的配偶子鞭打在 5 形成了并且释放天。在发展的开始, unicell 配偶子与异种极性把房间划分了成二,然后顶端的房间开发了初发的房间进 thalli,而基础房间发展成假根的初发的房间。在 8 天的文化,单音的原浆配偶子在长度发展成 240 μm 的少年细菌石楠。Unreleased 配偶子能在水藻身体以内直接发展。E。prolifera 也能通过除了显微镜的观察揭示的单性生殖的侧面的分叉或碎裂复制。在 E 的年老的织物上。prolifera 尽管降级的颜料部分留在褪色的海藻的细丝,众多的植物的房间能仍然在海藻的纸巾活跃地划分。 | 付刚 姚建亭 刘福利 刘吉东 王秀良 付万冬 李大鹏 周名江 孙松 段德麟 | 2008 | Chinese Journal of Oceanology and Limnology2008，26，4： | 6 |
| 4 | 羊栖菜“鹿丰1号”人工选育及养殖中试显示文摘为解决羊栖菜养殖生产需要的优良种苗,经过10多年努力,采用有性繁殖方式,进行定向选育,获得羊栖菜'鹿丰1号'。该优质羊栖菜的主要性状是:枝叶粗壮繁茂、气囊产生早、颗粒大、产量高,经过8年的养殖及检验,目标性状表现稳定,附苗总面积达到1912m2,培育出苗59.3hm2,总产量383.746t,平均产量6471kg/hm2。目前,该选育的羊栖菜已在温州地区的洞头县和苍南县以及山东部分海域进行了养殖中试,选育性状表现稳定,养殖产量高,为羊栖菜良种化的实现打下基础。 | 李生尧 许曹鲁 李建榜 姚建亭 刘吉东 段德麟 | 2010 | 渔业科学进展2010，31，2： | 5 |
| 5 | 中国近海重要生态建群红藻真江蓠的群体遗传多样性显示文摘真江蓠(Gracilaria vermiculophylla)是中国近海潮间带生态系统结构组成和功能维持的重要支撑物种,但有关其群体遗传结构和多样性分布模式的研究目前仍较缺乏。本研究利用线粒体cox1序列对我国近海19个真江蓠地理群体进行了系统发育和群体遗传分析。461个长度为641 bp的cox1序列片段共含有21个多态位点,产生15个单倍型。基于cox1序列的系统进化分析、单倍型分析和主成分分析显示,19个真江蓠群体分化为南北两个类群,其中浙江嵊泗以北的13个群体形成北方类群,福建厦门以南的6个群体形成南方类群。遗传距离和分子方差分析显示真江蓠南北各类群内的遗传分化较小,南北类群间的遗传分化达到亚种水平。南北类群间的差异是我国近海真江蓠群体遗传变异的主要来源。 | 刘若愚 孙忠民 姚建亭 胡自民 段德麟 | 2016 | 生物多样性2016，24，7： | 5 |
| 6 | 海带磷酸甘露糖变位酶(PMM)基因的克隆与表达分析显示文摘磷酸甘露糖变位酶(PMM)是褐藻胶和岩藻聚糖合成过程中的关键酶之一。本研究利用c DNA末端快速克隆(RACE)技术,获得2条海带PMM基因(Sjpmm1,Sjpmm2)序列。其中,Sjpmm1的开放阅读框(ORF)长759 bp,其编码的Sj PMM1为卤酸脱卤酶(HAD)超家族成员,含252个氨基酸,分子量约为28.51 k Da;而Sjpmm2的ORF长1866 bp,其编码的Sj PMM2属于磷酸己糖变位酶超家族的成员,含621个氨基酸,分子量约为66.49 k Da。海带PMM的二级结构均以?-螺旋为主。进化分析表明,Sjpmm1来自于原始真核生物,而Sjpmm2来源于质体的第一次内共生作用。实时定量PCR分析发现,海带受到高温或低温胁迫时,Sjpmm1和Sjpmm2转录水平上升,以合成岩藻聚糖抵抗环境影响。此外,利用p MAL-c5X载体对Sj PMM1进行体外表达,得到高浓度的可溶性融合蛋白,为后续的Sj PMM功能分析提供基础。 | 张朋艳 于雪 姚建亭 段德麟 | 2016 | 海洋科学2016，40，3： | 3 |
| 7 | Characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica(Laminariales,Phaeophyta)显示文摘Saccharina japonica is a common macroalga in sublittoral communities of cold seawater environments,and consequently may have highly effi cient ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)activity for carbon assimilation.In our study,we cloned the full-length Rubisco gene from S.japonica(SJ-rbc).It contained an open reading frame for a large subunit gene(SJ-rbcL)of 1 467 bp,a small subunit gene(SJ-rbcS)of 420 bp,and a SJ-rbcL /S intergenic spacer of 269 bp.The deduced peptides of SJ-rbcL and SJ-rbcS were 488 and 139 amino acids with theoretical molecular weights and isoelectric points of 53.97 kDa,5.81 and 15.84 kDa,4.71,respectively.After induction with 1 mmol/L isopropyl-β-Dthiogalactopyranoside for 5 h and purifi cation by Ni 2+ affi nity chromatography,electrophoresis and western blot detection demonstrated successful expression of the 55 kDa SJ-rbcL protein.Real-time quantitative PCR showed that the mRNA levels of SJ-rbcL in gametophytes increased when transferred into normal growth conditions and exhibited diurnal variations: increased expression during the day but suppressed expression at night.This observation implied that Rubisco played a role in normal gametophytic growth and development.In juvenile sporophytes,mRNA levels of SJ-rbcL,carbonic anhydrase,Calvin-BensonBassham cycle-related enzyme,and chloroplast light-harvesting protein were remarkably increased under continuous light irradiance.Similarly,expression of these genes was up-regulated under blue light irradiance at 350 μmol/(m 2·s).Our results indicate that long-term white light and short-term blue light irradiance enhances juvenile sporophytic growth by synergistic effects of various photosynthetic elements. | 邵展茹 刘福利 李秋莹 姚建亭 段德麟 | 2014 | Chinese Journal of Oceanology and Limnology2014，32，2： | 3 |
| 8 | 分子标记概述及其在藻类中的应用 | 王秀良 赵凤娟 吕慧 姚建亭 刘吉东 段德麟 | 2007 | 海洋科学集刊2007，，1： | 3 |
| 9 | 羊栖菜养殖品系DNA指纹图谱的研究显示文摘对羊栖菜(Hizikia fusiformis(Harv)Okamura)养殖中常见的3个品系进行了DNA指纹分析及遗传变异的研究,构建了其遗传指纹图谱,分析了不同种群的遗传关系,为羊栖菜的种质鉴定及选育提供了理论依据。运用RAPD分子标记技术,对5个羊栖菜的种群中共125个个体进行了分析,从300个引物中筛选出12条随机扩增引物共扩增135个位点,多态位点比率为84.4%。从中选择了4个多态性位点,构建了DNA指纹图谱。相关结果对羊栖菜遗传选育和种质鉴定等有参考价值。 | 吕慧 姚建亭 刘福利 王秀良 李生尧 段德麟 | 2009 | 海洋科学2009，33，12： | 2 |
| 10 | Early development of Costaria costata(C.Agardh) Saunders and cultivation trials显示文摘Costaria costata(C.Agardh) Saunders is one of common kelps distributed in many coastal areas worldwide;however,in China,no reports have been made on cultivation of the genus.To investigate potential cultivation of the species in the northern part of China,trials on isolation and preservation of the gametophytes were conducted using C.costata from Korea;growth and development of the gametophytes were observed.We showed that at 10±1°C,60 μmol m-2s-1 and 12:12 h(L:D),freshly released zoospores settled down within 1 hour,and then developed into the primary cell during the following 2 days.After a vegetative growth phase lasting 6-8 days,female gametophytes became 3-4 times larger in diameter than that of the primary cell,but still remained at a unicellular stage,while male gametophytes divided into 4-10 cells with only a slight change in size.Fertilization occurred within 10 days after the zoospores were released from the sporangia,and the apical and basal tissues of the juvenile sporophyte divided and differentiated into the blade and stipe.Temperature and irradiance influenced gametophytic vegetative growth and developmental patterns.Generally,low irradiance(15 μmol m-2s-1 and 30 μmol m-2s-1) was unfavorable to the induction of fertility,but it enhanced female gametophyte division.The optimal conditions for vegetative growth were 15°C and 30 μmol m-2s-1.After transplantation of the juvenile seedlings and after eight months cultivation,the harvested mature blade reached 194 cm in length and 32.7 cm in width.Our study proves that it is feasible to implement propagation and large scale cultivation of C.costata in northern China. | 付刚 刘吉东 王高歌 姚建亭 王秀良 段德麟 | 2010 | Chinese Journal of Oceanology and Limnology2010，28，4： | 2 |
| 11 | 海带转录组SSR序列特征及其相关基因功能分析显示文摘对海带(Saccharina japonica)转录组测序数据进行分析,从70 497条Unigenes中共检测到9 237个简单序列重复(SSR)位点,并对包含SSR序列的Unigenes进行功能注释。海带转录组中SSR的类型十分丰富,其中单核苷酸和三核苷酸重复SSR的数量最多,分别占SSR总数的40.9%和39.4%,其次为四核苷酸、二核苷酸、五核苷酸和六核苷酸重复,分别占SSR总数的9.8%,6.1%,3.1%和0.7%。SSR重复单元类型共有147种,其重复次数的范围为5~70次。功能注释发现约50%含有SSR的Unigenes获得了注释信息,并且大多数与已知蛋白有同源性。COG、GO功能分类结果均表明,大量含有SSR序列的基因与多种生物功能有关,其中与碳水化合物代谢及参与细胞组成相关的基因数量最多。本研究结果为深入开发功能性SSR标记奠定基础,也为开展海带分子标记辅助选育提供支持。 | 李秋莹 张杰 姚建亭 王秀良 段德麟 | 2016 | 海洋科学2016，40，11： | 1 |
| 12 | 关于整顿村容村貌的思考与建议显示文摘在社会主义新农村建设过程中,有些地区以改变村容村貌作为切入点,选择了部分村屯作为试点.开展了整修道路.解决饮水困难、改灶、改厕、整顿院落等方面的工作。 | 姚建亭 | 2007 | 建设科技2007，，7： | 1 |
| 13 | Population genetics of Sargassum horneri (Fucales, Phaeophyta) in China revealed by ISSR and SRAP markers显示文摘Sargassum horneri is a common brown macro-alga that is found in the inter-tidal ecosystems of China. To investigate the current status of seaweed resources and provide basic data for its sustainable development, ISSR (inter simple sequence repeat) and SRAP (sequence related amplified polymorphism) markers were used to analyze the population genetics among nine natural populations of S. horneri. The nine studied populations were distributed over 2 000 km from northeast to south China. The percentage of polymorphic loci P% (ISSR, 99.44%; SRAP, 100.00%), Nei's genetic diversity H (ISSR, 0.107-0.199; SRAP, 0.100-0.153), and Shannon's information index I (ISSR, 0.157-0.291; SRAP, 0.148-0.219) indicated a fair amount of genetic variability among the nine populations. Moreover, the high degree of gene differentiation G st (ISSR, 0.654; SRAP, 0.718) and low gene flow N m (ISSR, 0.265; SRAP, 0.196) implied that there was significant among-population differentiation, possibly as a result of habitat fragmentation. The matrices of genetic distances and fixation indices (F st ) among the populations correlated well with their geographical distribution (Mantel test R=0.541 5, 0.541 8; P=0.005 0, 0.002 0 and R=0.728 6, 0.641 2; P=0.001 0, 0.001 0, respectively); the Rongcheng population in the Shandong peninsula was the only exception. Overall, the genetic differentiation agreed with the geographic isolation. The fair amount of genetic diversity that was revealed in the S. horneri populations in China indicated that the seaweed resources had not been seriously affected by external factors. | 于深辉 崇卓 赵凤娟 姚建亭 段德麟 | 2013 | Chinese Journal of Oceanology and Limnology2013，31，3： | 1 |
| 14 | Formation and early development of tetraspores of Polysiphonia urceolata (Rhodomelaceae,Rhodophyta)显示文摘Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18°C, 25 μmol photons m-2 s-1 in 12:12 h (light:dark) photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions. | 姚建亭 李大鹏 于深辉 刘吉东 段德麟 | 2009 | Chinese Journal of Oceanology and Limnology2009，27，2： | 1 |
| 15 | 海带CRY-DASH基因的克隆与转录表达分析显示文摘利用cDNA末端快速克隆(RACE)技术,获得海带(Saccharina japonica)CRY-DASH基因(SjCRYDASH)全长序列,结构分析发现,其ORF区长1779bp,编码592个氨基酸。进行氨基酸同源序列比对,其与其他藻类和高等植物间存在两个重要辅基MTHF和FAD结合的保守域。通过不同光质照射诱导海带幼孢子体,发现蓝光、白光诱导1h后,均能使SjCRY-DASH转录水平上升,且SjCRY-DASH对蓝光的响应更强烈。本研究结果为研究大型褐藻-海带CRY-DASH受光诱导调控功能打下基础。 | 李璐 张朋艳 姚建亭 段德麟 | 2019 | 海洋科学2019，43，4： | 0 |
| 16 | Long interspersed elements in three species of Gracilaria (Gracilariaceae, Rhodophyta)显示文摘In order to find out whether long interspersed elements (LINEs) existed in macro-algae ge- nomes or not, we tested the LINE homologues in representative families (species): Gracilaria (G. eucheumoides Harv., G. tenuistipitata Chang et Xia, and G. textorii (Sur) De-Toni), Laminaria (L. longis- sima Miyabe and L. japonica Aresch.), and Ulva (U. lactuca L. and U. pertusa Kjellm.) during 2004 to 2005. Polymerase Chain Reaction (PCR) was carried out with degenerate oligonucleotide primers de- signed from LINEs of rice homologues and Cin4 of maize. Cloning and nucleotide sequencing of the PCR products revealed that 4 clones that derived from 3 species of Gracilaria have LINE homologues. The nucleotide sequences of the 4 LINE homologues diverged greatly, but the amino acid sequences deduced from them were relatively conserved. The endonuclease regions of the LINE homologues greatly di- verged from that of other plants, but they had closer phylogenetic relationship to Zepp elements in Chlor- ella sp., which indicated that sequence divergence by vertical transmission has been a major influence on the evolution of algal LINEs. | 李玉晖 姚建亭 高天翔 段德麟 | 2007 | Chinese Journal of Oceanology and Limnology2007，25，1： | 0 |
| 17 | New record of Lobophora rosacea(Dictyotales; Phaeophyceae) from the South China Sea显示文摘Lobophora rosacea C.W. Vieira, Payri et De Clerck is reported from the South China Sea for the first time. Our specimens are very similar to L. rosacea recently described from New Caledonia, not only in morphology but also in rbcL and cox3 gene sequences. The fan-shaped thallus grows erectly, attaching to the substrate by a basal holdfast. The thallus is composed of a single layer of large medullary cells and three to four layers of cortical cells on both sides of the medulla. Mature sporophytes are detected, with sporangium sori scattered on both surfaces of the thallus. | 孙忠民 王永强 严鹏程 郭辉 姚建亭 田中次郎 川井浩史 | 2017 | Chinese Journal of Oceanology and Limnology2017，35，1： | 0 |
| 18 | 海带热休克蛋白70基因体外原核表达研究显示文摘在前期研究克隆得到全长海带HSP70基因的基础上,为进一步研究藻类HSP70的生物学功能,将海带HSP70基因的开放阅读框区域克隆到表达载体pEASY-E2中,并转化到大肠杆菌BL21(DE3)pLysS。将阳性重组子培养于含有AMP(100 U/mL)的LB培养基,IPTG诱导表达,SDS-PAGE电泳鉴定。经5 h诱导,其表达量达到平台期,继续培养HSP70表达量并不显著增高。5 mM IPTG诱导海带HSP70蛋白表达量高于1 mM IPTG诱导蛋白表达量。 | 付万冬 姚建亭 段德麟 | 2011 | 浙江海洋学院学报(自然科学版)2011，30，5： | 0 |
| 19 | 切莫亏待了“铺路石”显示文摘科研院所转制是科技体制改革的重要内容,也是解放和发展生产力的重要举措,其成败与否直接关系到我国科技事业的发展、综合国力的增强和中华民族的兴盛.1999年中共中央和国务院发出<关于加强技术创新,发展高科技,实现产业化>的决定,为增强科技创新能力,应对知识经济挑战,建立具有中国特色的国家知识科技创新体系提出了明确方向. | 姚建亭 | 2002 | 团结2002，，4： | 0 |
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